七味白术散总苷对菌群失调腹泻小鼠肠道微生物及酶活性的影响  

Effects of Total Glycosides of Qiwei Baizhu Powder on Intestinal Microbiota and Enzyme Activities in Diarrhea Mice

作　　者：谢果珍[1] 唐圆 吴仪 黄莉莉 谭周进[1] XIE Guo-zhen;TANG Yuan;WU Yi;HUANG Li-li;TAN Zhou-jin(Hunan University of Chinese Medicine,Changsha 410208)

机构地区：[1]湖南中医药大学,长沙410208

出　　处：《生物技术通报》2021年第12期124-131,共8页Biotechnology Bulletin

基　　金：国家自然科学基金青年科学基金项目(81804076);湖南省自然科学基金青年项目(2020JJ5426);湖南省研究生科研创新项目(CX20200757)。

摘　　要：为揭示七味白术散治疗菌群失调腹泻的物质基础,从七味白术散中提取总苷,探讨了不同剂量的七味白术散总苷对菌群失调腹泻小鼠肠道微生态的影响。结果显示七味白术散总苷有助于恢复菌群失调腹泻小鼠的摄食量及体重。中剂量的七味白术散总苷使菌群失调腹泻小鼠的脾脏指数回到正常水平。不同剂量的七味白术散总苷均可促进双歧杆菌、乳酸菌及大肠杆菌的增殖,其中低剂量的七味白术散总苷对双歧杆菌(P<0.01)及乳酸菌(P<0.05)的增殖效果显著。不同剂量的七味白术散总苷可提高菌群失调腹泻小鼠肠道微生物活度(P<0.01)、乳糖酶(P<0.01)及蔗糖酶活性(P<0.01或P<0.05)。研究表明七味白术散总苷对菌群失调腹泻小鼠肠道有益菌、微生物活度及酶活性的影响与水煎液一致,是七味白术散治疗菌群失调腹泻的重要药效成分。To explore the material basis of Qiwei Baizhu Powder(QWBZP)in dysbacteria diarrhea treatment,the total glycosides(TG)from QWBZP was extracted,and the effects of TG on the intestinal microecology of diarrhea mice were investigated.The results showed that TG was conducive to restoring the food intake and body weight of diarrhea mice.TG in medium dose helped the spleen index restored to normal level.All doses of TG promoted the proliferation of Bifidobacterium,Lactobacillus and Escherichia coli,and TG in a low dose significantly promoted the proliferation of Bifidobacterium(P<0.01)and Lactobacillus(P<0.05).All doses of TG increased intestinal microbial activities(P<0.01),lactase activities(P<0.01)and sucrase activities(P<0.01 or P<0.05)in the mice with diarrhea.This study revealed that the effects of TG on beneficial bacteria,intestinal microbial activities and enzyme activities of the diarrhea mice were similar to that by the decoction of QWBZP.Glycosides are important pharmacological component of QWBZP in dysbacteria diarrhea treatment.

关 键 词：七味白术散总苷 肠道微生物 微生物活度 酶活性 

分 类 号：R285.5[医药卫生—中药学]