两种肾虚证型去势模型大鼠骨小梁微观结构及骨代谢的差异  

Differences of trabecular microstructure and bone metabolism in two types of kidney-deficiency ovariectomized rats

作　　者：何兴鹏 郑利钦 李鹏飞 悦桂阳 李志鸿 吴敏辉 林梓凌[5,6] He Xingpeng;Zheng Liqin;Li Pengfei;Yue Guiyang;Li Zhihong;Wu Minhui;Lin Ziling(First School of Clinical Medicine of Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;Jiangmen Traditional Chinese Medicine Hospital Affiliated to Jinan University,Jiangmen 529000,Guangdong Province,China;Jiangmen Central Hospital,Jiangmen 529030,Guangdong Province,China;International College of Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;Department of Traumatic Orthopedics,First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;Lingnan Medical Research Center of Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China)

机构地区：[1]广州中医药大学第一临床医学院,广东广州510405 [2]暨南大学附属江门中医院,广东江门529000 [3]江门市中心医院,广东江门529030 [4]广州中医药大学国际学院,广东广州510405 [5]广州中医药大学第一附属医院创伤骨科,广东广州市510405 [6]广州中医药大学岭南医学研究中心,广东广州510405

出　　处：《中国组织工程研究》2022年第23期3768-3772,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金面上项目(81673996),项目负责人:林梓凌;国家自然科学基金青年项目(81904220),项目负责人:李鹏飞。

摘　　要：背景:目前去势大鼠是成熟可靠的骨质疏松症动物模型,对肾阳虚证、肾阴虚证大鼠模型的建立与相关观察指标也已有比较详细的研究,但对肾阳虚证、肾阴虚证骨质疏松的“病证结合”大鼠模型研究还有待完善。交感-副交感神经功能测定是判断阴虚、阳虚证型的一个特异性指标,但用来分析两种肾虚证型骨骼系统相关指标方面仍存在不足,“病证结合”需要更多的实验依据和观察指标。目的:比较分析肾阳虚证和肾阴虚证骨质疏松大鼠骨小梁微观结构参数及血清中骨代谢指标的差异。方法:手术摘除20只SD雌性大鼠双侧卵巢建立去势骨质疏松模型,10周后随机分为肾阳虚组、肾阴虚组,每组10只。肾阳虚组去势大鼠臀部肌肉注射氢化可的松注射液,肾阴虚组去势大鼠灌胃甲状腺片混悬药液,均1次/d,连续14 d,造模成功后采用Micro-CT扫描获取大鼠左侧股骨感兴趣区域骨微结构参数,ELISA法检测大鼠血清中骨形成及骨吸收相关指标。结果与结论:①成功建立两种肾虚证型去势骨质疏松大鼠病证结合模型;②与肾阳虚组大鼠相比,肾阴虚组大鼠骨小梁骨密度及骨体积分数明显升高,骨小梁数量增加,骨小梁厚度减小,血清各项骨形成、骨吸收指标明显升高,提示肾阴虚组大鼠骨代谢过程明显加快;③结果表明,肾阳虚、肾阴虚证型去势骨质疏松大鼠骨小梁微观结构及骨代谢指标存在明显差异,其机制仍需进一步研究。BACKGROUND:At present,ovariectomized rats are a mature and reliable animal model of osteoporosis,and establishment and observational evaluation of rat models of two types of kidney deficiency have been relatively detailed.However,further exploration is needed on the rat models of osteoporosis combined with syndromes of kidney-yang deficiency and kidney-yin deficiency.The sympathetic-parasympathetic nerve function test is used as a specific index to judge the syndrome of yin-deficiency and yang-deficiency,but this indicator is relatively insufficient for evaluating and analyzing the skeletal system of the two types of kidney-deficiency syndromes.“Integration of disease and syndrome”requires more experimental evidence and observational indicators.OBJECTIVE:To compare trabecular microstructure parameters and serum bone metabolism indicators between osteoporosis rat models of kidney-yang deficiency and kidney-yin deficiency METHODS:The ovaries of 20 female Spraque-Dawley rats were surgically removed to make postmenopausal osteoporotic rat models.Ten weeks after operation,all model rats were randomly divided into two groups(n=10 per group):kidney-yang deficiency group and kidney-yin deficiency group.Gluteal intramuscular injection of hydrocortisone and intragastric administration of thyroid hormone tablets were given to the kidney-yang deficiency group and the kidney-yin deficiency group,respectively,once a day for 14 consecutive days.After modeling,micro-CT was used to detect trabecular microstructure parameters in the region of interest of the left femur,and ELISA was used to detect serum bone formation and bone resorption indicators.RESULTS AND CONCLUSION:Two types of kidney-deficiency osteoporosis rat models were successfully established.Compared with the kidney-yang deficiency group,bone mineral density,bone volume/total volume,and trabecular number were significantly increased,trabecular thickness decreased,and serum bone formation and bone resorption indicators significantly increased in the kidney-yin defi

关 键 词：肾虚证 去势大鼠 骨质疏松 MICRO-CT 骨代谢指标 

分 类 号：R445[医药卫生—影像医学与核医学] R-332[医药卫生—诊断学]