基于铜纳米簇荧光猝灭选择性检测盐酸阿霉素  

Selective Detection of Doxorubicin Hydrochloride Based on Fluorescence Quenching of Copper Nanoclusters

作　　者：焦婷 温昊翔 李忠平 JIAO Ting;WEN Hao-Xiang;LI Zhong-Ping(School of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006,China;Institute of Environmental Sciences,Shanxi University,Taiyuan 030006,China;School of Chemistry and Chemical Engineering,Taiyuan University of Technology,Taiyuan 030024,China)

机构地区：[1]山西大学化学化工学院,太原030006 [2]山西大学环境科学研究所,太原030006 [3]太原理工大学化学化工学院,太原030024

出　　处：《分析化学》2022年第2期235-243,共9页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21575083);晋中学院创新团队项目(No.jzxycxtd2019007);第“十二批”山西省百人计划项目(No.128,129)资助。

摘　　要：以聚乙烯亚胺(PEI)为稳定剂,抗坏血酸(AA)为还原剂,制备了铜纳米簇(PEI@CuNCs),并基于此建立了一种快速、简便的检测盐酸阿霉素(DOX)的方法。制备的PEI@CuNCs粒径约2.4 nm,最大激发波长为430 nm,最大发射波长为518 nm,具有良好的稳定性和抗光漂白性能。通过X射线光电子能谱以及傅里叶变换红外光谱等技术对PEI@CuNCs的表面官能团进行了表征。DOX可以猝灭PEI@CuNCs的荧光,因此将其作为荧光探针用于DOX的检测,在最优条件及1~145μmol/L浓度范围内,DOX的浓度与F/F_(0)(F和F_(0)分别为存在和不存在DOX时探针的荧光强度)具有良好的线性关系,检出限(S/N=3)为0.032μmol/L。DOX对探针的荧光猝灭机制为内滤效应(IFE),本方法对DOX具有较高的选择性。血清中DOX的加标回收率为95.6%~105.5%,表明本方法具有良好的实用性。本研究为检测DOX提供了一种新方法。A kind of novel copper nanoclusters(PEI@CuNCs)with sizes of about 2.4 nm was prepared using polyethyleneimine(PEI)as stabilizer and ascorbic acid as reducing agent,which could be used as probe to detect doxorubicin hydrochloride(DOX).The prepared PEI@CuNCs showed good stability and resistance to photobleaching,and their maximal excitation and emission wavelengths were 430 and 518 nm,respectively.The surface functional groups of PEI@CuNCs were characterized by X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy.DOX could quench the fluorescence of PEI@CuNCs,on the basis of this,a fluorescence method for detection of DOX was established.Under the optimal conditions,in the DOX concentration range of 1-145μmol/L,a good linear relationship between the concentration of DOX and F/F_(0)was observed,with a detection limit of 0.032μmol/L(S/N=3).In the presence of other antibiotics,this method had high selectivity to DOX.The fluorescence quenching of PEI@CuNCs by DOX was attributed to internal filtration effect(IFE).This work showed great prospects for detecting DOX.

关 键 词：铜纳米簇 荧光 聚乙烯亚胺 抗生素 盐酸阿霉素 

分 类 号：O657.3[理学—分析化学] TQ460.72[理学—化学]