基于基因组结构变异构建萝卜种质资源分子身份证  

Establishment of Molecular Identify Card for Radish Germplasm Resources Based on Genome Structural Variation

作　　者：严承欢 崔磊[1] 任志勇 黄燕[1,2] 矫振彪 朱凤娟[1] 张振兴[1] 甘彩霞 邓晓辉[1] 邱正明[1] YAN Chenghuan;CUI Lei;REN Zhiyong;HUANG Yan;JIAO Zhenbiao;ZHU Fengjuan;ZHANG Zhenxing;GAN Caixia;DENG Xiaohui;QIU Zhengming(Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement,Institute of Economic Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,Hubei,China;College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,Hubei,China)

机构地区：[1]湖北省农业科学院经济作物研究所,蔬菜种质创新与遗传改良湖北省重点实验室,湖北武汉430064 [2]华中农业大学园艺林学学院,湖北武汉430070

出　　处：《中国蔬菜》2022年第1期49-57,共9页China Vegetables

基　　金：国家重点研发计划项目(2021YFD1600303);国家大宗蔬菜产业技术体系项目(CARS-23-B06);国家自然科学基金项目(31902058);湖北省农业科学院青年科学基金项目(2020NKYJJ04)。

摘　　要：萝卜种质资源类型多样,难以通过表型评价判定其亲缘关系。本试验整合4份萝卜全基因组重测序数据和520份萝卜简化基因组测序数据进行生物信息学分析,鉴定了萝卜群体基因组水平的结构变异,开发了24个通用性的分子标记。使用上述标记对255份萝卜种质进行基因分型,构建了相应的萝卜分子身份证。聚类分析结果表明,在相关系数0.55处可将255份萝卜种质分为4个类群。基于基因组水平的大片段插入/缺失开发分子标记,具有通用性高、成本低、检测方便等优点,可以促进萝卜的种质纯度鉴定及分子育种工作。The types of radish germplasm resources are multiple.It is difficult to determine its genetic relationship by phenotype evaluation.This experiment integrated the whole genome resequencing data of 4 radish accessions and the reduced genomic sequencing data of 520 radish accessions to carry out bioinformatics analysis;identified the structural variation at genome-scale of radish population;and developed 24 userfriendly molecular markers.Using the above markers,this experiment conducted genotyping on 255 accessions of radish germplasm resources,and constructed corresponding radish molecular identity cards.Clustering analysis revealed that 255 accessions radish germplasm resources could be divided into 4 categories at the correlation coefficient of 0.55.Based on the klenow fragment insertion/deletion development of molecular markers at genome level,the advantages of high versatility,low cost,convenient for detection would promote the purity identification of radish germplasm resources and molecular breeding.

关 键 词：萝卜 结构变异 重测序 分子身份证 种质资源 

分 类 号：S631.1[农业科学—蔬菜学]