血清TIPE2在慢性肾小球肾炎中的表达和意义  

The Expression and Significance of Serum TIPE2 in Chronic Glomerulonephritis

作　　者：齐建军[1] 杨麦青 张林 汪钊 王艳峰 QI Jian-jun;YANG Mai-qing;ZHANG Lin;WANG Zhao;WANG Yan-feng(Department of Nephrology,Changyi People's Hospital,Changyi 261300,Shandong,China;Department of Pathology,Changyi People's Hospital,Changyi 261300,Shandong,China;Department of Pathology,Beidahuang Industry Group General Hospital,Harbin 150088,Heilongjiang,China)

机构地区：[1]昌邑市人民医院肾内科,山东昌邑261300 [2]昌邑市人民医院病理科,山东昌邑261300 [3]北大荒集团总医院病理科,黑龙江哈尔滨150088

出　　处：《医学信息》2022年第1期111-114,共4页Journal of Medical Information

基　　金：潍坊市卫生健康委员会科研项目(编号:WFWSJK-2021-261)。

摘　　要：目的分析慢性肾小球肾炎(CGN)外周血单个核细胞中肿瘤坏死因子-α诱导蛋白-8样分子-2(TIPE2)表达变化及在CGN诊断中的价值,并探讨其与血清转化生长因子-β1(TGF-β1)的相关性。方法选取2018年10月-2021年6月在昌邑市人民医院初次诊治经肾穿刺病理诊断符合CGN的患者45例,同时选取20例健康人设为对照组。应用PCR法检测外周血单个核细胞TIPE2的表达水平,采用酶联免疫吸附试验检测血清TGF-β1的水平,分析CGN常见临床病理因素,探讨TIPE2与临床病理因素及TGF-β1的相关性,并应用受试者操作特征(ROC)曲线评估血清TIPE2对CGN诊断的价值。结果CGN组TIPE2 mRNA相对表达量高于对照组,差异有统计学意义(P<0.05);CGN组血清尿素氮、肌酐、TGF-β1的表达水平高于正常对照组,差异有统计学意义(P<0.05);血清TIPE2 mRNA相对表达量的高低与患者的年龄、性别、BMI无关(P>0.05),与血清尿素氮、肌酐、TGF-β1的表达水平及CKD分期、肾间质损伤程度有关(P<0.05);Pearson相关性分析显示,TIPE2的相对表达量与血尿素氮、肌酐、TGF-β1的水平分别呈正相关(r=0.262、0.300、0.443,P=0.041、0.022、0.001);系膜增生性肾小球肾炎、膜性肾病、系膜毛细血管性肾小球肾炎和局灶性肾小球硬化4种病理类型分组之间TIPE2的表达比较,差异无统计学意义(P>0.05);ROC曲线结果表明,血清TIPE2用于诊断CGN的曲线下面积是0.739(P<0.05)。结论CGN患者血清TIPE2、TGF-β1的水平明显增高,TIPE2与TGF-β1的表达水平呈正相关,血清TIPE2用于诊断CGN的敏感性较高。Objective To analyze the expression changes of tumor necrosis factor-α-inducible protein-8-like molecule-2(TIPE2)in peripheral blood mononuclear cells of chronic glomerulonephritis(CGN)and its value in the diagnosis of CGN,and to explore its correlation with serum transforming growth factor-β1(TGF-β1).Methods From October 2018 to June 2021,45 patients with CGN diagnosed by renal biopsy in Changyi People's Hospital were selected,and 20 healthy people were selected as control group.The expression level of TIPE2 in peripheral blood mononuclear cells was detected by PCR,and the level of serum TGF-β1 was detected by enzyme-linked immunosorbent assay.The common clinicopathological factors of CGN were analyzed,and the correlation between TIPE2 and clinicopathological factors and TGF-β1 was discussed.The receiver operating characteristic(ROC)curve was used to evaluate the value of serum TIPE2 in the diagnosis of CGN.Results The relative expression of TIPE2 mRNA in CGN group was higher than that in control group,and the difference was statistically significant(P<0.05).The expression levels of serum urea nitrogen,creatinine and TGF-β1 in CGN group were higher than those in normal control group,and the difference was statistically significant(P<0.05).The relative expression level of serum TIPE2 mRNA was not correlated with age,gender and BMI(P>0.05),but was correlated with the expression levels of serum urea nitrogen,creatinine and TGF-β1,CKD stage and renal interstitial injury(P<0.05).Pearson correlation analysis showed that the relative expression of TIPE2 was positively correlated with blood urea nitrogen,creatinine and TGF-β1 levels(r=0.262,0.300,0.443,P=0.041,0.022,0.001).There was no significant difference in the expression of TIPE2 among the four pathological types of mesangial proliferative glomerulonephritis,membranous nephropathy,mesangial capillary glomerulonephritis and focal glomerulosclerosis(P>0.05).ROC curve results showed that the area under the curve of serum TIPE2 for diagnosis of CGN was 0.739(P<

关 键 词：慢性肾小球肾炎 肿瘤坏死因子-α诱导蛋白-8样分子-2 血清转化生长因子-Β1 血清尿素氮 肌酐 肾间质损伤 

分 类 号：R692.31[医药卫生—泌尿科学]