瘤背石磺体腔细胞分类、免疫相关酶特性及初步转录组学分析  

Study on cell classificatioin, immune-related enzymatic characteristics and preliminary transcriptome of coelomocytes in Onchidium struma

作　　者：吕庭莉 杲绍强 张虎[4] 张易婵 陈鹏 王嘉伟 乔帼 杨伯平[5] 张明明 Lü Tingli;Gao Shaoqiang;Zhang Hu;Zhang Yichan;Chen Peng;Wang Jiawei;Qiao Guo;Yang Boping;Zhang Mingming(College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China;College of Marine and Biology Engineering,Yancheng Institute of Technology,Yancheng 224051,China;Xinhe Town Agro-Tech Extension and Service Center,Xuzhou 221313,China;Jiangsu Marine Fisheries Research Institute,Nantong 226007,China;College of Electrical Engineering,Yancheng Institute of Technology,Yancheng 224051,China)

机构地区：[1]大连海洋大学水产与生命学院,辽宁大连116023 [2]盐城工学院海洋与生物工程学院,江苏盐城224051 [3]邳州市新河镇农业技术推广服务中心,江苏徐州221313 [4]江苏省海洋水产研究所,江苏南通226007 [5]盐城工学院电气工程学院,江苏盐城224051

出　　处：《海洋学报》2022年第2期84-93,共10页

基　　金：国家自然科学基金面上项目(32071615);江苏省水生生物资源重大专项(ZYHB16-5)。

摘　　要：瘤背石磺(Onchidium struma)广泛分布于潮间带和潮下带,是海洋生物向陆地生物辐射进化的重要代表,也是环境监测的指示生物。为丰富瘤背石磺的基础免疫学资料,本文研究了瘤背石磺体腔细胞的类别、细胞密度和体腔液免疫相关酶活性,并通过RNA-Seq测序获得体腔细胞转录本,进行生物信息学分析。结果表明,瘤背石磺主要有变形细胞、球形细胞和色素细胞3种不同类型的体腔细胞,其中,球形细胞数量最多,色素细胞数量最少;在无任何外源刺激下,体腔液内酸性磷酸酶、碱性磷酸酶、超氧化物歧化酶和酚氧化酶等抗氧化酶和免疫相关酶均具有活性,且超氧化物歧化酶和酚氧化酶活性最高;转录组学分析显示,体腔细胞中共组装出63097个Unigenes,KEGG将这些Unigenes主要注释到"信号转导""运输和分解代谢""内分泌系统""翻译""折叠、分选和降解"等信号通路。研究结果将利于进一步揭示瘤背石磺的体腔液及其体腔细胞在机体免疫防御过程中的作用,丰富瘤背石磺的基础免疫学资料。Onchidium struma is widely distributed in intertidal zone and subtidal zone, and considered as a representative species of evolution from ocean to land and an indicator organism for evaluation of environment quality.To enrich the basic immunological data of O. struma, the classification of coelomocytes, cell density and immunerelated enzyme activity of the coelomic fluid are studied, and also transcriptome analysis is performed by RNA-Seq sequencing. The results show that there are three different types of coelomocytes, including amoebocytes, spherulocytes and chromatocytes. The number of coelomocytes from more to less are spherulocytes,amoebocytes,chromatocytes. The higher enzyme activities including acid phosphatase(ACP), alkaline phosphatase(AKP), superoxide dismutase(SOD) and polyphenol oxidase(PO), are detected in coelomic fluids. The activities of SOD and PO are highest. Based on De novo transcriptome analysis, a total of 63 097 unigenes are assembled. KEGG annotations of these unigenes are mostly enriched in “signal transduction” “transport and catabolism” “endocrine system” “translation” “folding, sorting and degradation”, and signalling pathways. The present results will be helpful to further reveal the important role of the body cavity fluid and body coelomocytes in the host immune defense, and enrich the basic immunological data of O. struma.

关 键 词：瘤背石磺 体腔液 体腔细胞 免疫相关酶 转录组分析 

分 类 号：S917.4[农业科学—水产科学]