A single-cell morpho-transcriptomic map of brassinosteroid action in the Arabidopsis root  

作　　者：Moritz Graeff Surbhi Rana Jos R.Wendrich Julien Dorier Thomas Eekhout Ana Cecilia Aliaga Fandino Nicolas Guex George W.Bassel Bert De Rybel Christian S.Hardtke 

机构地区：[1]Department of Plant Molecular Biology,University of Lausanne,Biophore Building,1015 Lausanne,Switzerland [2]Ghent University,Department of Plant Biotechnology and Bioinformatics,Technologiepark 71,9000 Ghent,Belgium [3]VIB Center for Plant Systems Biology,Technologiepark 71,9000 Ghent,Belgium [4]Bioinformatics Competence Center,University of Lausanne,Genopode Building,1015 Lausanne,Switzerland [5]School of Life Sciences,The University of Warwick,Coventry,CV47AL,UK [6]Lead contact

出　　处：《Molecular Plant》2021年第12期1985-1999,共15页分子植物（英文版）

基　　金：funded by core funding from the University of Lausanne,the Swiss National Science Foundation(grant 310030B_185379,awarded to C.S.H.);The Research Foundation-Flanders(FWO,post-doc fellowship 1215820N,awarded to T.E.);the European Research Council(ERC Start ing Grant TORPEDO,714055,awarded to B.D.R.);the BBSRC(grant BB/S002804/1 to G.W.B.);the Deutsche Forschungsgemeinschaft(DFG,post-doctoral fellowship GR 5009/1-1,awarded to M.G.).

摘　　要：The effects of brassinosteroid signaling on shoot and root development have been characterized in great detail but a simple consistent positive or negative impact on a basic cellular parameter was not identified.In this study,we combined digital 3D single-cell shape analysis and single-cell mRNA sequencing to charac-terize root meristems and mature root segments of brassinosteroid-blind mutants and wild type.The resul-tant datasets demonstrate that brassinosteroid signaling affects neither cell volume nor cell proliferation capacity.Instead,brassinosteroid signaling is essential for the precise orientation of cell division planes and the extent and timing of anisotropic cell expansion.Moreover,we found that the cell-aligning effects of brassinosteroid signaling can propagate to normalize the anatomy of both adjacent and distant brassinosteroid-blind cells through non-cell-autonomous functions,which are sufficient to restore growth vigor.Finally,single-cell transcriptome data discern directly brassinosteroid-responsive genes from genes that can react non-cell-autonomously and highlight arabinogalactans as sentinels of brassinosteroid-dependent anisotropic cell expansion.

关 键 词：ARABIDOPSIS ROOT MERISTEM BRASSINOSTEROID single-cell mRNA sequencing segmentation morphology BRI1 digital single-cell analysis 

分 类 号：Q943.2[生物学—植物学]