An angiosperm NLR Atlas reveals that NLR gene reduction is associated with ecological specialization and signal transduction component deletion  

作　　者：Yang Liu Zhen Zeng Yan-Mei Zhang Qian Li Xing-Mei Jiang Zhen Jiang Ji-Hong Tang Dijun Chen Qiang Wang Jian-Qun Chen Zhu-Qing Shao 

机构地区：[1]State Key Laboratory of Pharmaceutical Biotechnology,School of Life Sciences,Nanjing University,Nanjing 210023,China [2]Jiangsu Key Laboratory for the Research and Utilization of Plant Resources,Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China

出　　处：《Molecular Plant》2021年第12期2015-2031,共17页分子植物（英文版）

基　　金：supported by the National Natural Science Foundation of China(32070243 to Z.Q.S.and 31770245 to J.Q.C.);the Fundamental Research Funds for the Central Universities(020814380169 to Z.Q.S.)。

摘　　要：Nucleotide-binding leucine-rich-repeat(NLR)genes comprise the largest family of plant disease-resis-tance genes.Angiosperm NLR genes are phylogenetically divided into the TNL,CNL,and RNL subclasses.NLR copy numbers and subclass composition vary tremendously across angiosperm genomes.However,the evolutionary associations between genomic NLR content and ecological adaptation,or between NLR content and signal transduction components,are poorly characterized because of limited genome avail-ability.In this study,we established an angiosperm NLR atlas(ANNA,https://biobigdata.nju.edu.cn/ANNA/)that includes NLR genes from over 300 angiosperm genomes.Using ANNA,we revealed that NLR copy numbers differ up to 66-fold among closely related species owing to rapid gene loss and gain.Interestingly,NLR contraction was associated with adaptations to aquatic,parasitic,and carnivorous life-styles.The convergent NLR reduction in aquatic plants resembles the lack of NLR expansion during the long-term evolution of green algae before the colonization of land.A co-evolutionary pattern between NLR subclasses and plant immune pathway components was also identified,suggesting that immune pathway deficiencies may drive TNL loss.Finally,we identified a conserved TNL lineage that may function independently of the EDS1-SAG101-NRG1 module.Collectively,these findings provide new insights into the evolution of NLR genes in the context of ecological adaptation and genome content variation.

关 键 词：NLR genes plant disease resistance EDS1 gene family gene family evolution ANGIOSPERMS 

分 类 号：Q943.2[生物学—植物学]