Coordinated regulation of plant immunity by poly(ADP-ribosyl)ation and K63-linked ubiquitination  

作　　者：Dongsheng Yao Marcus A.Arguez Ping He Andrew F.Bent Junqi Song 

机构地区：[1]Texas A&M AgriLife Research Center at Dallas,Texas A&M University System,Dallas,TX 75252,USA [2]Department of Biochemistry and Biophysics,Texas A&M University,College Station,TX 77843,USA [3]Department of Plant Pathology,University of Wisconsin-Madison,Madison,WI 53706,USA [4]Department of Plant Pathology and Microbiology,Texas A&M University,College Station,TX 77843,USA

出　　处：《Molecular Plant》2021年第12期2088-2103,共16页分子植物（英文版）

基　　金：supported by a start-up fund from Texas A&M AgriLife Research to J.S.;a grant from the National Science Foundation(IOS-1951094)to P.H.and J.S.

摘　　要：Poly(ADP-ribosyl)ation(PARylation)is a posttranslational modification reversibly catalyzed by poly(ADP-ribose)polymerases(PARPs)and poly(ADP-ribose)glycohydrolases(PARGs)and plays a key role in multi-ple cellular processes.The molecular mechanisms by which PARylation regulates innate immunity remain largely unknown in eukaryotes.Here we show that Arabidopsis UBC13A and UBC13B,the major drivers of lysine 63(K63)-linked polyubiquitination,directly interact with PARPs/PARGs.Activation of pathogen-associated molecular pattern(PAMP)-triggered immunity promotes these interactions and enhances PARylation of UBC13.Both parp1 parp2 and ubc13a ubc13b mutants are compromised in immune responses with increased accumulation of total pathogenesis-related(PR)proteins but decreased accu-mulation of secreted PR proteins.Protein disulfide-isomerases(PDIs),essential components of endo-plasmic reticulum quality control(ERQC)that ensure proper folding and maturation of proteins destined for secretion,complex with PARPs/PARGs and are PARylated upon PAMP perception.Significantly,PARylation of UBC13 regulates K63-linked ubiquitination of PDIs,which may further promote their disulfide isomerase activities for correct protein folding and subsequent secretion.Taken together,these results indicate that plant immunity is coordinately regulated by PARylation and K63-linked ubiquitination.

关 键 词：UBC13 PARP1 PARP2 PDI poly(ADP-ribosyl)ation K63-linked ubiquitination secretory pathway PAMP-triggered immunity systemic acquired resistance 

分 类 号：Q945[生物学—植物学]