CRISPR/Cas系统发展及其应用进展  

Development and application progress of CRISPR/Cas system

作　　者：张月婷 符潮 刘新亮 戴小英[1] 谢雄雄 汪信东[1] Zhang Yueting;Fu Chao;Liu Xinliang;Dai Xiaoying;Xie Xiongxiong;Wang Xindong(Jiangxi Academy of Forestry,Camphor Engineering and Technology Research Centre for National Forestry and Grassland Administration,Nanchang Jiangxi 330013,China)

机构地区：[1]江西省林业科学院·国家林业和草原樟树工程技术研究中心,江西南昌330013

出　　处：《南方林业科学》2021年第6期69-74,共6页South China Forestry Science

基　　金：江西省青年科学基金资助项目(项目编号:20202ACBL215003);江西省林业科学院公益项目(项目编号:2017512702)。

摘　　要：CRISPR/Cas系统作为当前最热门的编辑技术与其它基因编辑技术相比,具有操作简单、效率高等优点。目前该系统已被广泛应用于植物基因组编辑之中,为植物基因功能研究和遗传改良提供了重要的技术支持。本文首先介绍了CRISPR/Cas系统的发展历程,随后从两方面阐述了CRISPR/Cas系统的优化,最后着重介绍了CRISPR/Cas系统在植物中的发展方向及应用前景,以期为后续相关的遗传改良等研究提供参考。As the most popular editing technology,CRISPR/Cas system has the advantages of simple operation and high efficiency compared with other gene editing technologies.At present,the system has been widely used in plant genome editing,providing important technical support for plant gene function research and crop genetic improvement.This review firstly introduced the development process of the CRISPR/Cas system,then explained the optimization of the CRISPR/Cas system from two aspects,and finally focused on the development direction and application prospects of the CRISPR/Cas system in plants.It is hoped to provide reference for follow-up related genetic improvement research.

关 键 词：基因编辑技术 CRISPR/Cas系统 发展历程 遗传改良 

分 类 号：Q789[生物学—分子生物学]