急性呼吸窘迫综合征患者中性粒细胞基因表达谱的生物信息学分析  

Bioinformatics analysis of neutrophil gene expression profile in patients with acute respiratory disease syndrome

作　　者：王行行 戚迪[1] 何婧[1] 邓旺[1] 王导新[1] 赵燕[1] WANG Hanghang;QI Di;HE Jing;DENG Wang;WANG Daoxin;ZHAO Yan(Department of Respiratory and Critical Care Medicine,Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,P.R.China)

机构地区：[1]重庆医科大学附属第二医院呼吸与危重症医学科,重庆400016

出　　处：《中国呼吸与危重监护杂志》2021年第11期789-794,共6页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：重庆市自然科学基金面上项目(cstc2020 jcyjmsxmX0078)。

摘　　要：目的以生物信息学分析方法分析急性呼吸窘迫综合征(ARDS)中性粒细胞基因表达谱,以期找到新的治疗靶点。方法从基因表达芯片(GEO)数据库获得以ARDS患者和健康志愿者为试验对象的基因表达芯片,对高通量芯片数据进行提取,通过GEO2R、OmicsBean、STRING、Cytoscape等网站或软件筛选差异表达基因,并进一步在DAVID网站中进行基因本体论(GO)分析和京都基因与基因组百科全书(KEGG)通路富集分析。结果GEO2R网站筛选得到86个差异基因,STRING网站纳入其中81个基因进行蛋白互作分析,其结果经Cytoscape软件进一步分析得到11个枢纽基因:AHSP、ALAS2、CD177、CLEC4D、EPB42、GPR84、HBD、HVCN1、KLF1、SLC4A1和STOM。GO分析示差异基因多富集于细胞部位尤其是细胞膜的完整性上,KEGG分析示以细胞因子受体通路为主的多条通路参与ARDS发病。结论多种因素参与了ARDS的发病。本研究共筛选到可能参与ARDS发病的11个枢纽基因,可用于后续研究。Objective To explore the pathogenesis of acute respiratory disease syndrome(ARDS)by bioinformatics analysis of neutrophil gene expression profile in order to find new therapeutic targets.Methods The gene expression chips include ARDS patients and healthy volunteers were screened from the Gene Expression Omnibus(GEO)database.The differentially expressed genes were carried out through GEO2R,OmicsBean,STRING,and Cytoscape,then enrichment analysis of Gene Ontology(GO)and Kyoto Encyclopedia of Gene and Genomes(KEGG)pathways was conducted to investigate the biological processes involved in ARDS via DAVID website.Results Bioinformatics analysis showed 86 differential genes achieved through the GEO2R website.Eighty-one genes were included in the STRING website for protein interaction analysis.The results of the interaction were further analyzed by Cytoscape software to obtain 11 hub genes:AHSP,ALAS2,CD177,CLEC4 D,EPB42,GPR84,HBD,HVCN1,KLF1,SLC4A1,and STOM.GO analysis showed that the differential gene was enriched in the cellular component,especially the integrity of the plasma membrane.KEGG analysis showed that multiple pathways especially the cytokine receptor pathway involved in the pathogenesis of ARDS.Conclusions A variety of genes and pathways have been involved in the pathogenesis of ARDS.Eleven hub genes are screened,which may be involved in the pathogenesis of ARDS and can be used in subsequent studies.

关 键 词：急性呼吸窘迫综合征 生物信息学分析 枢纽基因 

分 类 号：R563.8[医药卫生—呼吸系统]