大气颗粒物暴露对大鼠心脏和肺组织的影响  

Effects of particulate matter exposure on heart and lung tissues of rats

作　　者：王念 郑钰凡 黄少坚 辜淦斌 徐阳 何芳 WANG Nian;ZHENG Yufan;HUANG Shaojian;GU Ganbin;XU Yang;HE Fang(School of Basic Medical Science,Guangzhou Medical University,Guangzhou,Guangdong 511436,P.R.China;School of Public Health,Guangdong Medical University,Dongguan,Guangdong 523000,P.R.China;School of Clinical Medicine,Guangzhou Medical University,Guangzhou,Guangdong 511436,P.R.China;College of Life Sciences,Guangzhou Medical University,Guangzhou,Guangdong 511436,P.R.China)

机构地区：[1]广州医科大学基础医学院,广东广州511436 [2]广东医科大学公共卫生学院,广东东莞523000 [3]广州医科大学临床医学院,广东广州511436 [4]广州医科大学生命科学院,广东广州511436 [5]中山大学中山医学院,广东广州510080

出　　处：《中国呼吸与危重监护杂志》2021年第11期795-801,共7页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：广东省医学科研基金(A2018272);广东省自然科学基金面上项目(2019 A1515011430)。

摘　　要：目的研究对来自机动车尾气(MVE)和生物燃料烟雾(BMF)这两种大气颗粒物(PM)暴露对大鼠心脏和肺组织的影响。方法将30只大鼠随机分为3组,分别暴露于清洁空气(对照组)、MVE(MVE组)和BMF(BMF组)各12周,通过组织和细胞形态学观察、马松染色和免疫组织化学等方法研究PM慢性暴露对心脏和肺组织形态结构的影响。结果大气PM暴露12周后,与对照组大鼠相比,MVE组和BMF组大鼠的心脏和肺组织均出现了细胞变性,间质炎性细胞数量增多,纤维组织增生,以及细胞凋亡增加等现象。结论暴露于大气PM 12周可对大鼠的心脏和肺组织产生明显的炎症和损伤。Objective To observe the effects of atmospheric particulate matter(PM)exposure from two sources of motor vehicle exhaust(MVE)and biomass fuel(BMF)on the heart and lung tissues of rats.Methods Thirty rats were randomly divided into 3 groups,and they were exposed to clean air(control group),MVE(MVE group)and BMF(BMF group)for 12 weeks,respectively.The effect of long-term PM exposure on the morphological structure of heart and lung tissues was investigated by tissue and cell morphological observations,Masson staining,and immunohistochemistry.Results Compared with rats in the control group,the rats in the MVE group and the BMF group showed cellular degeneration,increased number of interstitial inflammatory cells,fibrous tissue proliferation,and cell apoptosis in the heart and lung tissues of the PM-exposed rats.Conclusion Exposure to air pollution particulate matter for 12 weeks can significantly cause inflammatory and damage to the heart and lung tissues of rats.

关 键 词：大气颗粒物 机动车尾气 生物燃料烟雾 大鼠 心脏 肺 

分 类 号：R12[医药卫生—环境卫生学]