代谢组学揭示水稻——稻瘿蚊互作的潜在生化标识物  

Metabolomics reveals potential biomarkers of rice gall midge(Orseolia oryzae)interactions

作　　者：陈灿[1] 张宗琼[1] 夏秀忠[1] 杨行海[1] 农保选[1] 张晓丽[1] 徐志健[1] 李丹婷[1] 郭辉[1] 冯锐[1] CHEN Can;ZHANG Zong-qiong;XIA Xiu-zhong;YANG Xing-hai;NONG Bao-xuan;ZHANG Xiao-li;XU Zhi-jian;LI Dan-ting;GUO Hui;FENG Rui(Rice Research Institute,Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding,Nanning 530007,China)

机构地区：[1]广西农业科学院水稻研究所/广西水稻遗传育种重点实验室,南宁530007

出　　处：《南方农业学报》2021年第10期2662-2670,共9页Journal of Southern Agriculture

基　　金：国家自然科学基金地区科学基金项目(31560385);广西科技计划项目(桂科AB16380138,桂科AD18281067);广西自然科学基金项目(2018GXNSFAA138015)。

摘　　要：【目的】筛选稻瘿蚊(RGM)侵染下抗、感水稻材料差异代谢物,揭示水稻抗虫靶标化合物,为解析植物与昆虫互作关系及水稻害虫绿色防控提供理论依据。【方法】分别对抗RGM水稻材料GXM-001-2和易感RGM水稻材料TN1进行RGM接虫胁迫,接虫后48 h利用液相色谱—质谱联用仪(LC-MS)对RGM侵染及未侵染抗、感水稻材料的代谢物进行化学成分分析。【结果】RGM侵染下抗、感水稻材料的差异代谢物分析显示,LC-MS共检测到330种代谢物,其中155种为已知代谢物;被RGM侵染后,GXM-001-2中上调代谢物种类远少于其下调代谢物种类,也远少于TN1中的上调代谢物种类。RGM侵染下水稻潜在生化标识物种类分析表明,差异代谢物分为抗性特性(n=16)、易感特性(n=16)、侵染特性(n=7)和宿主特性(n=3)等4类。生化标识物及差异倍数分析表明,抗性特性物包括1-氨基环丙烷羧酸、3-磷酸甘油酸和二十烷酸等物质,其中6-甲基维生素D、谷氨酸、叶绿醇和酪氨酸等物质上调表达;易感特性物包括2-己酮酸、3-苯基乳酸和6-羟基己酸等物质,其中6-羟基己酸、葡萄糖和白皮杉醇等7种物质下调表达;侵染特性物包括γ-氨基丁酸、半乳糖酸和丙醇二酸等物质,其中腺嘌呤、尿苷、丙醇二酸和γ-氨基丁酸在抗、感材料中变化趋势一致;宿主特性物包括异亮氨酸、十六烷酸和环亮氨酸,前2种物质在GXM-001-2中显著高于TN1(P<0.05)。【结论】RGM侵染后从抗、感水稻材料中筛选到抗性特性物、易感特性物、侵染特性物和宿主特性物,其中脂肪酸及其衍生物(十六烷酸、二十烷酸、十四烷酸和β-羟基十四烷酸)、氨基酸(谷氨酸、酪氨酸、苯丙氨酸和γ-氨基丁酸)、酚类(阿魏酸)、萜类(叶绿醇)和胺类(乙酰苯胺、苄胺和乳酰胺)可能在植物抗虫防御反应中发挥重要作用,是RGM侵染水稻最重要的潜在生化标识物。【Objective】To detect differential levels of metabolites between rice gall midge(RGM)-resistant and-susceptible rice materials to provide a theoretical basis for analyzing rice-RGM interaction and assist in the development of green controls of RGM in rice.【Method】GXM-001-2(high resistance)and TN1(high susceptibility)were inoculated with RGM and liquid chromatography-mass spectrometry was employed to detect changes in metabolite levels after 48 h.【Result】330 metabolites were detected,of which 155 were known metabolites. TN1 displayed a much higher number of up-regulated metabolites after RGM infection than in GXM-001-2,where metabolites were largely down-regulated. The analysis of potential biochemical markers of rice infected by RGM showed that differentially regulated metabolites could be classified into four categories associated with resistance(n=16),susceptibility(n=16),infection(n=7)and host characteristics(n=3). Biochemical markers and fold change analysis showed that the resistance category included 1-aminocyclopropanicarboxylic acid,3-phosphoglyceric acid,eicosanoic acid,6-methylvitamin D,glutamic acid,phyllol and tyrosine of which,the latter 4 were upregulated. Compounds associated with susceptibility included 2-hexketoic acid,3-phenyllactic acid,6-hydroxy hexanoic acid and other substances,of which 7 substances,including 6-hydroxy hexanoic acid,glucose and piceatannol,were downregulated. The characteristic compounds of infection included 4-aminobutyric acid,galactoic acid,propanedioic acid and adenine,uridine,propanedioic acid,and γ-aminobutyric acid showed similar up or down trends in GXM-001-2 and TN1. The host characteristic substances consisted of isoleucine,hexadecanoic acid and cycloleucine,with the former two substances at significantly higher levels in GXM-001-2 relative to TN1(P<0.05).【Conclusion】Under rice RGM condition,16,16,7 and 3 metabolites associated with resistance,susceptibility,infection and host characteristics,respectively,were selected. Of these,fatty acids and thei

关 键 词：水稻 稻瘿蚊 代谢组 生化标识物 抗虫 

分 类 号：S511[农业科学—作物学]