聚乙二醇对微柱凝胶法检测不规则抗体的增强作用分析  

Enhancement Effect of Polyethylene Glycol on Irregular Antibody Detection by Microcolumn Gel Test

作　　者：刘晓骅 罗圆圆[1] 赵嫚 李族军[2] 汪德清[1] 练正秋[2] LIU Xiao-hua;LUO Yuan-yuan;ZHAO Man(Department of Blood Transfusion Medicine,First Medical Center,Chinese PLA General Hospital 100853)

机构地区：[1]解放军总医院第一医学中心输血医学科,100853 [2]成都市第三人民医院输血科

出　　处：《临床输血与检验》2022年第1期67-70,共4页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的探讨聚乙二醇-微柱凝胶法(PEG-MGT)检测不规则抗体的增强作用,及其对不规则抗体鉴定的适用情况。方法回顾性分析2018年4月~2021年4月低离子强度盐溶液-微柱凝胶法(LISS-MGT)凝集弱造成抗体鉴定不明确而加做PEG-MGT的70例标本,比较两种方法的16系谱细胞反应格局和实验结果。结果PEG-MGT检出不规则抗体29例(41.4%):Rh系统相关抗体20例(69%),Duffy系统相关抗体5例(17.2%),Kidd系统相关抗体3例(10.3%)和Lewis系统相关抗体1例(3.4%)。格局不明确,特异性待定标本41例(58.6%),对比LISS-MGT和PEG-MGT反应强度,PEG-MGT增强16例,减弱17例,反应强度无变化8例;通过盐水介质或4℃孵育微柱凝胶法可确定抗体特异性共13例,分别为抗-M抗体9例(PEG-MGT增强4例,减弱3例,强度保持不变4例),抗-P1抗体2例(PEG-MGT均增强),抗-Le;抗体(PEG-MGT增强)和抗-Le;抗体(PEG-MGT减弱)各1例。结论PEG-MGT可显著增强Rh系统、Duffy系统和Kidd系统的抗原抗体反应,对MNS系统、Lewis系统、P1系统等抗体的判断也有一定参考意义。Objective To explore the enhancement effect of polyethylene glycol microcolumn gel method(PEG-MGT)in irregular antibody detection.Methods We retrospectively analyzed 70 specimens with weak agglutination by low ionic strength salt solution-microcolumn gel method(LISS-MGT)as well as additional PEG-MGT results from April 2018 to April 2021 and compared the 16-pannel cell reaction pattern and experimental results.Results We identified the specific antibodies in 29 cases by PEG-MGT(41.4%),including 20 cases of Rh system-related antibodies(69%),5 cases of Duffy system antibodies(17.2%),3 cases of Kidd system antibodies(10.3%)and one case of Lewis system antibodies(3.4%).There were still 41 cases(58.6%)to be determined the specificity of antibodies.By comparing with the reaction intensity of LISS-MGT and the PEG-MGT results,the experimental results showed that PEG-MGT enhanced the reaction intensity in 16 cases,weakened in 17 cases,and unchanged in 8 cases.The antibodies could be determined by saline medium or MGT incubating at 4℃in a total of 13 cases,including 9 cases of anti-M antibodies(4 cases of PEG-MGT enhancement,3 cases of weakening,4 cases of constant strength),2 cases of anti-P1 antibody(both PEG-MGT enhancement),and anti-Le;antibody(PEG-MGT enhancement)and anti-Le;antibody(PEG-MGT weakening)one case each.Conclusion PEG-MGT method could significantly enhance the antigen-antibody response in Rh system,Duffy system and Kidd system,and it also could be helpful to determine antibodies of MNS system,Lewis system and P1 system.

关 键 词：PEG LISS 微柱凝胶法 抗体鉴定 

分 类 号：R446.6[医药卫生—诊断学]