苦竹叶多糖体外抗肿瘤活性研究  

Study on the Antiproliferative Effect of Polysaccharide from Pleioblastus Amarus Leaves

作　　者：潘静[1] 杨人泽[1] 钟斌[1] PAN Jing;YANG Renzhe;ZHONG Bin(First Affiliated Hospital of Gannan Medical University,Ganzhou Jiangxi 341000,China.)

机构地区：[1]赣南医学院第一附属医院,江西赣州341000

出　　处：《药品评价》2021年第23期1432-1435,共4页Drug Evaluation

基　　金：江西省中医药管理局课题(2019A272)。

摘　　要：目的:探讨苦竹叶多糖对肿瘤细胞增殖的抑制作用。方法:采用MTT法检测不同浓度的苦竹叶多糖对子宫颈癌HeLa细胞、肺癌A549细胞、人胃癌SGC7901三种常见的人恶性肿瘤细胞生长抑制率。结果:苦竹叶多糖在浓度为100、200、400、600μg/mL时对子宫颈癌HeLa细胞(P<0.05)、肺癌A549细胞(P<0.05)、人胃癌SGC7901细胞(P<0.05)有明显抑制作用。在浓度为50μg/mL时,对子宫颈癌HeLa细胞(P<0.05)、人胃癌SGC7901细胞(P<0.05)有一定的抑制作用,对肺癌A549细胞几乎无作用。在浓度为600μg/mL作用96 h后,对人胃癌SGC7901细胞增殖抑制作用最强,其IC_(50)值为386μg/mL。结论:苦竹叶多糖对此三种人恶性肿瘤细胞株的生长在一定的浓度范围内均有抑制作用,且呈剂量和时间依赖关系,此研究为推进苦竹叶化学成分基础研究和促进苦竹叶资源的综合利用提供了依据。Objective:To discuss the antiproliferative effect of polysaccharide from pleioblastus amarus leaves.Methods:MTT assay was employed to test the antiproliferative effect of polysaccharide from pleioblastus amarus leaves in three kinds of malignant tumor cell lines.Results:Polysaccharide from pleioblastus amarus leaves at 100μg/mL、200μg/mL、400μg/mL,600μg/mL inhibited the proliferation of Hela(P<0.05),A549(P<0.05),SGC7901(P<0.05)cell lines effectively.Polysaccharide from pleioblastus amarus leaves at 50μg/mL inhibited the proliferation of Hela(P<0.05),SGC7901(P<0.05)cell lines,but no effect on A549 cell.Polysaccharide from pleioblastus amarus leaves at 600μg/mL showed antitumor the best effects on the SGC7901 cell and IC_(50) was 386μg/mL.Conclusion:Polysaccharide from pleioblastus amarus leaves can inhibit three kinds of malignant tumor celllines,dose and time-dependent in a certain concentration range.This study provides references for promoting the basic research and comprehensive utilization of pleioblastus amarus leaves.

关 键 词：多糖类 苦竹叶 子宫肿瘤 A549细胞 胃肿瘤 抗肿瘤 

分 类 号：R285[医药卫生—中药学]