京研绿翡翠黄瓜品种纯度的SSR鉴定  

SSR purity identification of cucumber variety Jingyan Lvfeicui

作　　者：孟淑春[1] 徐秀苹[2] 刘立功[1] 宋顺华[1] 张峰[1] MENG Shu-chun;XU Xiu-ping;LIU Li-gong;SONG Shun-hua;ZHANG Feng(Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)/Key Laboratory of Urban Agriculture(North),Ministry of Agriculture and Rural Affairs,Beijing 100097,China;State Key Laboratory of Systematic and Evolutionary Botany,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China)

机构地区：[1]北京市农林科学院蔬菜研究中心/农业农村部华北地区园艺作物生物学与种质创制重点实验室/农业农村部都市农业(北方)重点实验室,北京100097 [2]中国科学院植物研究所系统与进化植物学国家重点实验室,北京100093

出　　处：《湖北农业科学》2022年第1期98-101,共4页Hubei Agricultural Sciences

基　　金：北京市农林科学院蔬菜研究中心改革与发展项目(KYCX202005-02);北京市农林科学院科技创新能力建设专项(KJCX20200113);北京市果菜创新团队岗位专家项目(GCTDZJ2014033002)。

摘　　要：为了确保种子遗传质量,准确高效地鉴定杂交黄瓜种子的品种纯度,用63对SSR引物对黄瓜(Cucumis sativus L.)品种京研绿翡翠及其亲本进行多态性筛选。结果表明,16对引物在亲本间具有多态性,并都表现出共显性,在杂交后代中表现为清晰、稳定的父母本互补的带型,特异性强,可以用来区别其中混杂的母本、父本及其他品种的种子。这16对SSR引物作为京研绿翡翠及其亲本的核心引物,构成京研绿翡翠的SSR指纹图谱,为京研绿翡翠杂交种的真伪鉴定、纯度检测及亲本提纯等工作提供准确的技术信息。其中10对引物扩增的条带可分辨程度高,利用这10对引物对京研绿翡翠黄瓜种子进行检测,其鉴定结果与田间鉴定结果一致。For ensuring the seed genetic quality,increasing the efficiency of application of the variety identification and seed genetic purity on cucumber hybrid seed,alternative 63 pairs of SSR primers have been applied in the screening the molecular markers on the cucumber Jingyan Lvfeicui and their parents.The results showed that 16 pairs of primers complementary to appear polymorphic bands between the parents,clear and stabilized,performing codominance,highly specificity,among hybrids,which can be used to distinct its parents and other varieties.The core SSR primers of Jingyan Lvfeicui were composed mainly of these 16 pairs of primers,formed the SSR fingerprint of Jingyan Lvfeicui,which provided technological information for seeds purity,authenticity of hybrid and parents purifying.Among them,the bands amplified by 10 pairs of primers were high distinguishable,the particularity was strong,as well as the characteristics of clear bands and the result agreed with field test.

关 键 词：京研绿翡翠黄瓜 SSR 纯度鉴定 

分 类 号：S642.2[农业科学—蔬菜学]