心脏死亡大鼠双肾移植模型的建立  

Establishment of a Model of Double Kidney Transplantation in Rats with Cardiac Death

作　　者：邓妙文 金钟大 DENG Miaowen;JIN Zhongda(Guangzhou U-niversity of Chinese Medicine,Guangdong 510000,China)

机构地区：[1]广州中医药大学第二临床医学院,510000 [2]广州中医药大学第二附属医院,510000

出　　处：《医学研究杂志》2022年第1期29-32,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81373523)。

摘　　要：目的探讨心脏死亡大鼠双侧供肾左侧原位肾移植模型的手术方法。方法供、受体均采用雄性近交系Wistar大鼠,分别于心脏死亡后0、20和40min取双侧供肾,行左侧原位肾移植术后摘除受体右肾,观察各组受鼠术后的移植肾的功能恢复情况及生存率。结果0min和20min组的大鼠移植肾功能良好、存活率高,40min组的大鼠移植肾功能较差且均于14天后死亡。0min组的左右供肾移植后的肾功能及存活率差异无统计学意义(P>0.05)。结论建立心脏死亡大鼠双侧供肾左侧原位肾移植模型稳定可靠,可有效减少供肾冷缺血时间及节约供体数量,可应用于心脏死亡供体肾移植实验研究。Objective To explore a surgical method of left orthotopic kidney transplantation model with bilateral donor kidneys in rats with cardiac death.Methods Both donor and recipient were male inbred Wistar rats.Donor kidneys were taken at 0min,20min and 40min after cardiac death,and left orthotopic kidney transplantation was performed,and the recipient′s right kidney was removed.The survival of the recipient mice in each group and the recovery of the transplanted kidney function were observed.Results Rats in group 0min and 20min had good kidney transplant function and high survival rate,while rats in group 40min had poor kidney transplant function and all died 14 days later.There was no statistical difference in the transplantation function and survival rate between the bilateral donors in the group 0min(P>0.05).Conclusion The left orthotopic kidney transplantation model of bilateral donor kidney in rats with cardiac death is stable and reliable,which can effectively reduce the cold ischemia time of donor kidney and save the number of donors,and can be applied to the experimental study of cardiac death donor kidney transplantation.

关 键 词：心脏死亡 肾移植 大鼠 模型 

分 类 号：R617[医药卫生—外科学]