两种诱变处理下紫花苜蓿全基因组DNA甲基化图谱分析  

Analysis of DNA Methylation Map of Alfalfa Whole Genome under Two Kinds of Mutagenesis Treatments

作　　者：申晓慧 SHEN Xiao-hui(College of Life Sciences,Resources and Environment Sciences,Jiangxi Province,Yichun University, Yichun, Jiangxi Province 336000, China)

机构地区：[1]宜春学院,生命科学与资源环境学院,江西宜春336000

出　　处：《草地学报》2022年第1期46-54,共9页Acta Agrestia Sinica

基　　金：博士科研启动项目(210-3360119010)资助。

摘　　要：为了探究不同诱变处理对紫花苜蓿表观遗传学效应的影响,试验采用甲基磺酸乙酯(EMS)和^(60)CO-γ两种诱变方法对紫花苜蓿种子进行处理,采用重亚硫酸盐测序(BS-seq)技术,对紫花苜蓿诱变前后全基因组DNA甲基化图谱进行分析。结果表明,紫花苜蓿体内的DNA甲基化主要有mCG,mCHH和mCHG 3种类型;C位点发生甲基化的比率在EMS诱变处理下表现为上升,在^(60)CO-γ诱变处理下表现为下降。此外,DNA甲基化主要发生在CG序列;对不同诱变处理组的差异甲基化区域(DMR)鉴定发现有105个基因在两组比较中均存在DMR,KEGG通路富集分析表明,在DMR相关基因中显著富集的通路主要包括氮代谢通路(Nitrogen metabolism)、淀粉和蔗糖代谢途径(Starch and sucrose metabolism)、脯氨酸代谢(Proline metabolism)等生物学过程。因此,诱变改变了苜蓿DNA甲基化水平,为诱变突变体的筛选提供了丰富材料。To investigate the epigenetic effects of different mutagenic treatments on alfalfa,ethyl methanesulfonate(EMS)and ^(60)Co-γwere used to treat alfalfa seeds.The whole-genome DNA methylation map of alfalfa before and after mutation was analyzed by bisulfite-sequencing(BS-seq).The results showed that there were three types of DNA methylation in alfalfa:mCG、mCHH and mCHG.The methylation rate of C site increased under EMS mutagenesis and decreased at ^(60)Co-γmutagenesis.In addition,DNA methylation mainly occurred in CG sequence.The identification of differential methylation region(DMR)of different mutation treatment groups found that 105 genes had DMR in two groups.KEGG pathway enrichment analysis showed that the pathways significantly enriched in DMR related genes mainly included nitrogen metabolism pathway,starch,and sucrose metabolism pathway,and proline metabolism processes.Therefore,mutagenesis changed the DNA methylation level of alfalfa and provided abundant materials for the screening of mutagenic mutants.

关 键 词：紫花苜蓿 DNA甲基化 重亚硫酸盐测序 表观遗传 

分 类 号：S816.4[农业科学—饲料科学]