外源ABA对镉胁迫下柳枝稷生理特性及镉吸收的影响  

Effects of Exogenous ABA on Physiological Characteristics and Cadmium Uptake of Switchgrass under Cadmium Stress

作　　者：肖霄 方志刚 纪燕玲[1] 娄来清[1] 常明 蔡庆生[1] XIAO Xiao;FANG Zhi-gang;JI Yan-ling;LOU Lai-qing;CHANG Ming;CAI Qing-sheng(College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095 China;College of Life and Geographic Sciences, Kashi University, Kashi, Xinjiang Province 844000, China)

机构地区：[1]南京农业大学生命科学学院,江苏南京210095 [2]喀什大学生命与地理科学学院,新疆喀什844000

出　　处：《草地学报》2022年第1期118-124,共7页Acta Agrestia Sinica

基　　金：国家自然科学基金(31372359);江苏省社会发展科技项目(BE2014709)资助。

摘　　要：为探究外源脱落酸(Abscisic acid,ABA)缓解柳枝稷(Panicum virgatum)镉(Cd)毒害效应及可能产生的生理机制,本试验以2个柳枝稷品种‘Alamo’与‘Cave in Rock’为材料,在水培条件下,研究了外源1μmol·L^(-1)ABA预处理对20μmol·L^(-1)镉(Cd^(2+))胁迫下柳枝稷幼苗生物量、根系形态、膜脂过氧化物及脯氨酸等指标的影响。结果表明:Cd显著抑制柳枝稷生长,‘Cave in Rock’比‘Alamo’的耐Cd性强,但‘Alamo’具有较高的Cd积累量及转运能力。与单独Cd胁迫相比,1μmol·L^(-1)ABA预处理可显著增加柳枝稷根长、根表面积和根尖数,提高根系活力、脯氨酸(Proline,Pro)及叶片相对含水量,降低植株中丙二醛(Malondialdehyde,MDA)含量,显著促进‘Alamo’对Cd的吸牧。根据本试验结果,在Cd污染土地上,推荐种植柳枝稷‘Alamo’品种,并用低浓度ABA处理,有利于促进植株生长的同时,提高Cd污染土壤的修复效率。To investigate the effects of exogenous ABA(Abscisic acid,ABA)on alleviating cadmium toxicity in switchgrass(Panicum virgatum)and its possible physiological mechanisms,two switchgrass varieties‘Alamo’and‘Cave in Rock’were used in the experiment.The effects of 1μmol·L^(-1)(ABA)pretreatment on biomass,root morphology,membrane lipid peroxidation and proline of switchgrass seedlings under hydroponic culture conditions with addition 20μmol·L^(-1) Cd^(2+) were investigated.The results showed that Cd significantly inhibited the growth of switchgrass.‘Cave in Rock’was more tolerant to Cd than‘Alamo’,but‘Alamo’had a higher Cd accumulation and translocation capacity than‘Cave in Rock’.Compared with Cd stress alone,1μmol·L^(-1)ABA pretreatment significantly increased root length,root surface area and root tip number,increased root vigour,proline(Pro)and relative water content of leaves,reduced malondialdehyde(MDA)content in the plant and promoted Cd uptake of‘Alamo’.The results suggest that planting‘Alamo’and treating it with low concentrations of ABA is recommended on Cd-contaminated land to promote plant growth while improving the remediation of Cd-contaminated soil.

关 键 词：脱落酸 柳枝稷 镉 生理机制 修复 

分 类 号：Q945[生物学—植物学]