玉米种子萌发特性对不同浓度PEG-6000的响应  

Response of Maize Seed Germination Characteristics to Different Concentrations PEG-6000

作　　者：黄爱花[1] 邹成林[1] 苏义成 翟瑞宁 莫润秀[1] 黄开健[1] 谭华[1] HUANG Ai-hua;ZOU Cheng-lin;SU Yi-cheng;ZHAI Rui-ning;MO Run-xiu;HUANG Kai-jian;JAN Hua(Maize Research Institute,Guangxi Academy of Agricultural Sciences,Guangxi Nanning 530007,China;Guangxi Academy of Agricultural Sciences,Guangxi Nanning 530007,China)

机构地区：[1]广西农业科学院玉米研究所,广西南宁530007 [2]广西区农业科学院,广西南宁530007

出　　处：《西南农业学报》2021年第12期2628-2633,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家重点研发计划项目(2018YFD0100105,2016 YFD01011206-6);广西重点研发计划项目(桂科AB18221056);广西农业科学院基本科研业务专项(桂农科2020YM89)。

摘　　要：【目的】分析不同浓度聚乙二醇6000(PEG-6000)对玉米萌发特性的影响,为广西抗旱玉米新品种选育提供参考依据。【方法】以玉米品种桂单162为试验材料进行PEG-6000模拟干旱胁迫,测定分析其在不同浓度[0(对照,CK)、5%、10%、15%、20%、25%和30%]PEG-6000溶液处理下的发芽势、发芽率、萌发指数、胚根长、胚芽长、幼苗全长、胚根干重、胚芽干重和贮藏物质运转率等萌发指标。【结果】随着PEG-6000浓度的提高,玉米种子的发芽势、发芽率、萌发指数、胚根长、胚芽长、幼苗全长、胚根干重、胚芽干重和贮藏物质运转率均呈逐渐下降趋势。当PEG-6000浓度为25%时,上述各指标较CK分别极显著下降77.03%、68.24%、75.52%、73.04%、83.86%、77.51%、89.67%、92.64%和91.68%(P<0.01,下同),与PEG-6000浓度为30%时的相应指标相比均无显著差异(P>0.05),但与PEG-6000浓度为20%时相比除胚芽长外均显著(P<0.05)或极显著低于其他相应的指标。相关分析结果表明,PEG-6000浓度与种子发芽势、发芽率、萌发指数、胚根长、胚芽长、幼苗全长、胚根干重、胚芽干重和贮藏物质运转率均呈极显著负相关,相关系数分别为-0.985、-0.886、-0.971、-0.975、-0.980、-0.990、-0.978、-0.978和-0.979。【结论】不同浓度PEG-6000干旱胁迫对玉米种子发芽势、发芽率、萌发指数、胚根长、胚芽长、幼苗全长、胚芽干重、胚根干重和贮藏物质转运率等具有明显的抑制作用,提高PEG-6000浓度会延缓玉米种子发芽时间,降低发芽能力,限制其胚根和胚芽的生长发育,使种子的贮藏物质运转率降低。在玉米干旱胁迫研究中建议选用的PEG-6000浓度为15%或20%。【Objective】The effects of different concentrations of polyethylene glycol 6000(PEG-6000)on maize germination characteristics were analyzed to provide a reference basis for the breeding of drought resistant new maize varieties in Guangxi.【Method】Using maize variety Guidan 162 as experimental material,PEG-6000 simulated drought stress was carried out,analyzed maize in different concentrations of[0(control,CK),5%,10%,15%,20%,25%and 30%]PEG-6000 solution under the process of germination potential,germination rate,germination index,radicle length,plumule length,seedling length,radicle dry weight of radicle,dry weight of plumule and storage material transfer rate.【Result】With the increase of PEG-6000 concentration,the germination potential,germination rate,germination index,radicle length,plumule length,seedling length,dry weight of radicle,dry weight of plumule and storage material transfer rate of maize decreased gradually.When the concentration of PEG-6000 was 25%,the above indexes extremely significantly(P<0.01,the same as below)decreased by 77.03%,68.24%,75.52%,73.00%,83.86%,77.51%,89.67%,92.64%and 91.68%respectively compared with CK.There was no significant difference compared with the corresponding indexes when the concentration of PEG-6000 was 30%(P>0.05),but compared with the concentration of PEG-6000 was 20%,they were significantly(P<0.05)or extremely significant lower than other corresponding indexes except length of plumule.The results of correlation analysis showed that PEG-6000 concentration had extremely significant negative correlation with germination potential,germination rate,germination index,radicle length,plumule length,seedling length,dry weight of radicle,dry weight of plumule and storage material transfer rate,and the correlation coefficients were-0.985,-0.886,-0.971,-0.975,-0.980,-0.990,-0.978,-0.978 and-0.979,respectively.【Conclusion】Drought stress with different concentrations of PEG-6000 had obvious inhibitory effects on maize seed germination potential,germination rate,germ

关 键 词：玉米种子 聚乙二醇(PEG) 干旱胁迫 萌发特性 

分 类 号：S513[农业科学—作物学]