香蕉系统获得抗性相关基因对外源水杨酸的响应分析  

Analysis of Expression of Systemic Acquired Resistance-related Genes in Banana Induced by Exogenous Salicylic Acid

作　　者：段雅婕[1] 郭志祥[2] 曾莉[2] 李舒 刘立娜 胡会刚[1] 李伟明[1] 白亭亭[2] DUAN Yajie;GUO Zhixiang;ZENG Li;LI Shu;LIU Lina;HU Huigang;LI Weiming;BAI Tingting(South Asia Tropical Crops Research Institute,Chinese Academy of Tropical Agriculture Sciences/Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture and Rural Affairs/Key Laboratory of Hainan Province for Postharvest Physiology and Tech-nology of Tropical Horticultural Products,Zhanjiang,Guangdong 524091,China;Agricultural Environment and Resources Insti-tute,Yunnan Academy of Agricultural Sciences,Kunming,Yunnan 650205,China)

机构地区：[1]中国热带农业科学院南亚热带作物研究所/农业农村部热带果树生物学重点实验室/海南省热带园艺产品采后生理与保鲜重点实验室,广东湛江524091 [2]云南省农业科学院农业环境资源研究所,云南昆明650205

出　　处：《热带作物学报》2022年第1期9-18,共10页Chinese Journal of Tropical Crops

基　　金：财政部和农业农村部国家现代农业产业技术体系项目(No.CARS-31-08);西藏自治区自然科学基金项目(No.XZ 2018ZR G-46);广东省自然科学基金项目(No.2020A1515010166)。

摘　　要：香蕉生产受多种病虫害和逆境胁迫的影响,由真菌病原引起的香蕉枯萎病、叶斑病和黑星病,细菌性病害软腐病和鞘腐病,以及非生物胁迫寒害等,是阻碍香蕉绿色可持续生产的严重问题。为探索香蕉生产上多种病害和寒害逆境的有效防控措施,本研究从外源水杨酸(SA)诱导植物系统抗性机理出发,通过实时荧光定量PCR(RT-qPCR)方法,分析外源水杨酸对香蕉系统抗性相关基因的诱导表达情况。结果显示,外源水杨酸能诱导感病品种‘巴西蕉’和抗病品种‘农科1号’香蕉植株内水杨酸合成途径关键基因显著上调表达,‘巴西蕉’中SK基因的相对表达量为对照的1.5倍以上,而‘农科1号’中该基因的相对表达量为对照的30倍以上;PAL基因在施用SA的‘巴西蕉’和‘农科1号’中整体表现出显著上调的趋势,在SA处理的‘农科1号’中,最高显著上调表达12.5倍,最低显著上调表达1.4倍,而在‘巴西蕉’中,最高显著上调表达3.1倍,最低显著上调表达1.7倍,SK和PAL 2个基因在抗病品种‘农科1号’中的上调幅度远远高于感病品种‘巴西蕉’;SA对CS和ICS基因的诱导上调幅度低于3倍,但在抗病品种‘农科1号’中的上调幅度仍高于感病品种‘巴西蕉’。信号传导途径转录因子NPR1和TGA、PR1基因显著上调表达,并且‘农科1号’比‘巴西蕉’诱导效果更明显。通过测定水杨酸处理的香蕉抗病相关基因对尖孢镰刀菌古巴专化型热带4号生理小种(Fusarium oxysporum f. sp. cubense tropical race 4, TR4)的响应情况,结果表明,TR4接种3 d可抑制‘巴西蕉’中多数PAL、NPR1和PR1基因的表达,但不会抑制‘农科1号’中多数PAL、NPR1和PR1基因的表达;在SA和TR4双重作用时,2个香蕉品种中的PAL、NPR1和PR1抗病相关基因被强烈诱导上调表达,PAL和POD防御酶活性显著增强。表明外源水杨酸具有诱导香蕉系统抗性抵御多种生物和非生物�Banana production is affected by a variety of diseases, pests and abiotic stresses. Banana Fusarium wilt, sigatoka and black spot caused by fungal pathogens, soft rot and sheath rot caused by bacteria, abiotic stresses such as cold damage, are serious problems hindering banana green sustainable production. In order to explore effective prevention and control measures for a cultivar of diseases and cold stress in banana production, this study started from the mechanism of exogenous salicylic acid inducing plant system resistance, and analyzed the induced expression of exogenous salicylic acid(SA) on banana systemic resistance-related genes by the real-time fluorescent quantitative PCR(RT-qPCR) method. Results showed that exogenous SA could induce significant up-regulation of key genes in the SA synthesis pathway in banana plants. The relative expression level of SK gene in SA-treated susceptible ‘Brazilian’ was more than 1.5 folds than that in the control ‘Brazilian’, while the relative expression level of this gene in SA-treated tolerant ‘Nongke No. 1’ was more than 30 folds than that in the control ‘Nongke No. 1’. The PAL genes showed a significant up-regulation trend in both of SA-treated ‘Brazilian’ and ‘Nongke No. 1’. In the SA-treated ‘Nongke No. 1’, the highest significantly up-regulated expression of PAL was 12.5 folds, and the lowest significantly up-regulated expression was 1.4 folds. While in SA-treated ‘Brazilian’, the highest significantly up-regulated expression of PAL was 3.1 folds and the lowest significantly up-regulated expression was 1.7 folds. The up-regulation of SK and PAL genes in the tolerant cultivar ‘Nongke No. 1’ treated with SA was much higher than that in the susceptible cultivar ‘Brazilian’ treated with SA. SA induced up-regulation of CS and ICS genes by no more than 3 folds, but it still showed that the up-regulation in the tolerant cultivar ‘Nongke No. 1’ was higher than that in the susceptible cultivar ‘Brazilian’. NPR1,TGA, and PR1 g

关 键 词：香蕉 诱导抗性 抗性基因 生物与非生物胁迫 

分 类 号：S668.1[农业科学—果树学]