二氧化碳在华北大黑鳃金龟幼虫定位寄主植物根系中的作用  

The role of carbon dioxide in the orientation of Holotrichia oblita larvae to the host plant root

作　　者：刘香雨 赵旭 王志敏 秦健辉 屈亚飞 李克斌[1] 曹雅忠[1] 尹姣[1] LIU Xiangyu;ZHAO Xu;WANG Zhimin;QIN Jianhui;QU Yafei;LI Kebin;CAO Yazhong;YIN Jiao(State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《植物保护》2022年第1期29-36,共8页Plant Protection

基　　金：国家重点研发计划-政府间国际科技创新合作重点专项(2017YFE0130400);北京市自然科学基金(6212026)。

摘　　要：为探究二氧化碳(CO_(2))在华北大黑鳃金龟Holotrichia oblita幼虫定位寄主植物根系时所发挥的作用,对其开展了触角电位生理反应及行为生测试验。研究结果表明,CO_(2)浓度为0.75%、1.5%、3%、6%时均能显著激发幼虫的触角电位和趋性反应,且随着浓度增加幼虫对CO_(2)的趋向爬行所用时间显著缩短;根系分泌物同样能引起华北大黑鳃金龟幼虫的触角电位和趋性行为反应,且与1.5%CO_(2)间无显著差异;将1.5%CO_(2)分别与棕榈酸甘油酯、2-丁烯酸、肉豆蔻酸、十四烷、4-甲基-2-氧戊酸、十二烷、环己六醇、邻二甲苯和对羟基苯甲酸等9种根系分泌物混合后对试虫引诱效果增强,其趋向爬行时间显著缩短。以上结果显示,CO_(2)在华北大黑鳃金龟幼虫定位寄主植物根系过程中发挥了重要的指引和增效作用,试验结果可为华北大黑鳃金龟幼虫的绿色防治提供新思路。In order to explore the role of carbon dioxide(CO_(2))in locating the roots of host plants by the larvae of Holotrichia oblita,the electroantennogram(EAG)and behavioral responses were assayed.The results showed that the stimulation of 0.75%,1.5%,3%,6%CO_(2)elicited a strong reaction of the larvae,and their crawling speed became faster with increasing concentration of CO_(2).The root exudates could also induce an EAG and chemotaxis response of H.oblita larvae,and the results were similar to that of the group of 1.5%CO_(2).Furthermore,mixing 1.5%CO_(2)with nine kinds of root exudates,i.e.,tripalmitin,2-butenoic acid,myristic acid,tetradecane,4-methyl-2-oxovaleric acid,dodecane,inositol,o-xylene and p-hydroxybenzoic acid,respectively,could enhance the attraction effect and significantly shorten the crawling time.These results suggested that CO_(2)played an important role in guiding and enhancing the locating capability of the larvae of H.oblita for the host plant roots,which provides a new idea for green control of H.oblita larvae.

关 键 词：华北大黑鳃金龟幼虫 二氧化碳 植物根系分泌物 触角电位 行为反应 

分 类 号：S433.5[农业科学—农业昆虫与害虫防治]