AsSod基因调控茄链格孢致病性及对胁迫的响应  

AsSod gene regulates the pathogenicity of Alternaria solani and its response to stress

作　　者：赵冬梅[1] 侯晓丹 东曼 白家琪 范莎莎 杨志辉[1] 朱杰华[1] ZHAO Dongmei;HOU Xiaodan;DONG Man;BAI Jiaqi;FAN Shasha;YANG Zhihui;ZHU Jiehua(College of Plant Protection, Hebei Agricultural University, Baoding 071000, China)

机构地区：[1]河北农业大学植物保护学院,保定071000

出　　处：《植物保护》2022年第1期37-43,共7页Plant Protection

基　　金：河北省教育厅青年基金(QN2019156);国家现代农业产业技术体系建设专项(CARS-09-P18)。

摘　　要：由茄链格孢Alternaria solani引起的马铃薯早疫病是马铃薯生产上的重要病害之一,可导致马铃薯大面积减产,进而造成巨大的经济损失。本研究在获得缺失AsSod基因的茄链格孢突变株(ΔAsSod)的基础上,对ΔAsSod进行了不同的胁迫处理。结果表明,相比于野生型菌株和回复株,突变株ΔAsSod对细胞壁胁迫因子SDS以及渗透胁迫因子KCl、NaCl、山梨醇的耐受性均减弱。进一步分析发现,ΔAsSod对外源过氧化物胁迫更敏感,胞内过氧化物酶和漆酶活性均显著降低。在对突变株孢子萌发和致病力的研究中发现,突变株ΔAsSod的孢子萌发率显著低于野生型菌株和回复株。同时,突变株ΔAsSod的致病力也显著降低,表明AsSod基因在茄链格孢对外界胁迫的耐受性、孢子萌发和致病性方面发挥重要的作用。Potato early blight caused by Alternaria solani is one of the most important diseases in potato production,which can greatly reduce the yield of potato.In the previous study,we obtained an AsSod deletion mutant of A.solani(ΔAsSod),and different stress treatments were carried out onΔAsSod.It was found that the tolerance ofΔAsSod to cell wall stress factors SDS and osmotic stress factors KCl,NaCl and sorbitol was weaker than that of wild and complementation strains.Furthermore,it was found thatΔAsSod was more sensitive to exogenous peroxide stress,and the activities of intracellular peroxidase and laccase inΔAsSod decreased significantly.The spore germination rate ofΔAsSod was significantly lower than that of wild and complementation strains.At the same time,the pathogenicity of theΔAsSod also decreased significantly,indicating that the AsSod plays an important role in the tolerance to external stress,spore germination and pathogenicity of A.solani.

关 键 词：马铃薯早疫病 茄链格孢 超氧化物歧化酶(SOD) 致病性 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]