烟粉虱中甘薯双生病毒(sweepoviruses)半巢式PCR快速检测技术的建立与应用  

Development of semi-nested PCR method for rapid detection of sweepoviruses in Bemisia tabaci

作　　者：秦艳红[1] 王爽[1] 田雨婷[1] 乔奇[1] 张德胜[1] 王永江[1] 张振臣[1] QIN Yanhong;WANG Shuang;TIAN Yuting;QIAO Qi;ZHANG Desheng;WANG Yongjiang;ZHANG Zhenchen(Institute of Plant Protection, Henan Academy of Agricultural Sciences, Henan Key Laboratory of Crop Pest Control, IPM Key Laboratory in Southern Part of North China for Ministry of Agricultural and Rural Affairs, Zhengzhou 450002, China)

机构地区：[1]河南省农业科学院植物保护研究所,河南省农作物病虫害防治重点实验室,农业农村部华北南部作物有害生物综合治理重点实验室,郑州450002

出　　处：《植物保护》2022年第1期168-172,共5页Plant Protection

基　　金：国家甘薯产业技术体系(CARS-10-B13)。

摘　　要：甘薯双生病毒(sweepoviruses)是侵染甘薯的一类重要病毒,通过烟粉虱以持久方式传播,我国甘薯上至少存在8种甘薯双生病毒。本研究根据我国已报道的8种甘薯双生病毒基因组保守区设计了一组引物,建立了单头烟粉虱中甘薯双生病毒的半巢式PCR快速检测方法。特异性和灵敏性分析结果表明,半巢式PCR具有较高的特异性和灵敏性,以8种不同甘薯双生病毒重组质粒为模板时,该方法能同时检测出8种甘薯双生病毒,且半巢式PCR方法的灵敏性比常规PCR高10~10000倍。对烟粉虱的检测结果表明,该方法能稳定地从单头烟粉虱中检测出甘薯双生病毒。该方法具有经济、快速,灵敏性、特异性和稳定性均较好等优点。Sweepoviruses are the viruses infecting sweet potato and transmitted by Bemisia tabaci in persistent manner.At least eight viruses had been identified in Chinese sweet potato.Primers were designed based on the conserved regions of genome of eight sweepoviruses from China.The rapid semi-nested PCR detection technology was established for the detection of sweepoviruses in single whitefly.The semi-nested PCR had high specificity and sensitivity.With the recombination plasmids of eight sweepoviruses as template,eight viruses could be simultaneously detected.The sensitivity of the semi-nested PCR was higher than that of conventional PCR by 10-10000 times.Sweepoviruses could be steadily detected from individual whitefly by using the semi-nested PCR assay.This assay is of economical,rapid,sensitive,specific and stable for rapid detection of sweepoviruses in B.tabaci.

关 键 词：甘薯双生病毒 半巢式PCR 检测 

分 类 号：S435.31[农业科学—农业昆虫与害虫防治]