LED红光对糖尿病大鼠创面愈合的影响  

Effects of LED red light on wound healing in diabetic rats

作　　者：李明[1,2] 刘甜甜[1,2] 朱含汀 方勇 倪涛[1,2] Li Ming;Liu Tiantian;Zhu Hanting;Fang Yong;Ni Tao(Department of Plastic and Reconstructive Surgery, Shanghai Ninth People′s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China;Institute of Trauma, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China)

机构地区：[1]上海交通大学医学院附属第九人民医院整复外科,200011 [2]上海交通大学医学院创伤研究所,200011

出　　处：《中华损伤与修复杂志（电子版）》2022年第1期18-24,共7页Chinese Journal of Injury Repair and Wound Healing(Electronic Edition)

基　　金：上海市临床重点专科建设项目-整形外科(shslczdzk00901);上海交通大学医学院附属第九人民医院交叉基金(JYJC202101)。

摘　　要：目的探讨LED红光照射对糖尿病大鼠创面愈合的作用及相关机制。方法将30只4周龄雄性SD大鼠按随机数字表法分为正常创面组、糖尿病创面组、红光治疗糖尿病创面组,每组10只。红光治疗糖尿病创面组及糖尿病创面组大鼠高脂饮食4周,正常创面组大鼠正常饮食。饲养4周后对红光治疗糖尿病创面组及糖尿病创面组大鼠按50 mg/kg的量腹腔注射10 mg/mL的链脲佐菌素(STZ)制作糖尿病大鼠模型,2组大鼠均造模成功。造模成功后在3组大鼠的背部两侧各制造2个1.5 cm×1.5 cm的全层皮肤缺损创面,每2 d对大鼠创面进行酒精消毒1次,红光治疗糖尿病创面组大鼠每次消毒后对创面进行LED红光照射5 min,能量密度为20 J/cm2,另外2组大鼠不进行LED红光照射。在观察第7、10、14、21天,观察红光治疗糖尿病创面组大鼠创面有无出现皮疹、红肿、水疱、烫伤等光照不良反应;肉眼观察3组大鼠创面愈合情况;统计3组大鼠创面愈合率。观察第10天从各组随机取2只大鼠,处死后取背部创面组织,固定后,进行苏木精-伊红染色观察创面新生血管情况及肉芽组织生长情况;采用免疫荧光法检测各组大鼠创面组织中CD34、血管内皮细胞生长因子(VEGF)表达情况。数据比较采用单因素方差分析和LSD-t检验。结果观察第7、10、14、21天,红光治疗糖尿病创面组大鼠经LED红光照射后皮肤未见皮疹、红肿、水疱、烫伤等光照不良反应。在各个观察时间点,肉眼观察正常创面组及红光治疗糖尿病创面组创面愈合情况均优于糖尿病创面组,且正常创面组创面愈合情况略优于红光治疗糖尿病创面组;观察第7、10、14、21天,正常创面组的创面愈合率分别为(34.62±2.116)%、(53.83±7.92)%、(70.20±5.41)%、(95.65±2.58)%,红光治疗糖尿病创面组创面愈合率分别为(31.76±2.44)%、(50.48±4.54)%、(66.26±11.35)%、(93.96±2.80)%,糖尿病创面组创面愈合率分Objective To investigate the effect and related mechanism of LED red light irradiation on wound healing in diabetic rats.Methods Thirty 4-week-old male SD rats were divided into 3 groups,normal wound group,diabetic wound group,and diabetic wound treated with red light group according to the random number table method,with 10 rats in each group.The rats in diabetic wound treated with red light group and diabetic wound group had a high-fat diet for 4 weeks,and the rats in the normal wound group had a normal diet.After 4 weeks,the rats in the diabetic wound treated with red light group and the diabetic wound group were intraperitoneally injected with 10 mg/mL Streptozotocin(STZ)at a dose of 50 mg/kg to make diabetes models and both groups of rats were successfully modeled.After successful modeling,two full-thickness skin defect wounds of 1.5 cm×1.5 cm were made on both sides of the backs of the three groups of rats,and the wounds of the rats were disinfected with alcohol every two days,and the wounds of the rats in the diabetic wound treated with red light group were irradiated with LED red light at energy density of 20 J/cm2 for 5 minutes after the disfection,the other two groups of rats were not irradiated with LED red light.On the 7th,10th,14th,and 21st days of observation,whether there were rashes,redness,blisters,scalds and other adverse light reactions on the wounds of the rats in the diabetic wound treated with red light group were observed,the wound healing condition of the three groups of rats was observed by naked eye,and the wound healing rate of the three groups of rats was counted.On the 10th day of observation,2 rats were randomly selected from each group,and the back wound tissue was taken after being sacrificed.After fixation,hematoxylin-eosin staining was performed to observe the condition of new blood vessels and the growth of granulation tissue on the wound surface;the expressions of CD34 and vascular endothelial growth factor(VEGF)in wound tissue of rats in each group were detected by immunofluo

关 键 词：创伤和损伤 大鼠 糖尿病 伤口愈合 光生物调节治疗 慢性创面 

分 类 号：R47[医药卫生—护理学]