基于网络药理学研究妇科千金片/胶囊治疗盆腔炎性疾病后遗症的作用机制  

The mechanism of Fuke Qianjin Tablet/Capsule in treating sequelae of pelvic inflammatory disease based on network pharmacology

作　　者：吴梦瑶 张鹏 凌勇根 郑元青 白璐 赵毅 章泽恒[2] WU Mengyao;ZHANG Peng;LING Yonggen;ZHENG Yuanqing;BAI Lu;Zhao Yi;ZHANG Zeheng(Zhuzhou Qianjin Pharmaceutical Co.,Ltd.,Zhuzhou,Hunan 412000,China;Hunan Provincial Center for Drug Evaluation,Certification and Adverse Reaction Monitoring,Changsha,Hunan 410013,China)

机构地区：[1]株洲千金药业股份有限公司,湖南株洲412000 [2]湖南省药品审评认证与不良反应监测中心,湖南长沙410013

出　　处：《湖南中医药大学学报》2022年第2期213-222,共10页Journal of Hunan University of Chinese Medicine

基　　金：湖南省科药联合基金项目(S2021JJKYLH0081);国家中药标准化项目(ZYBZH-C-HUN-21)。

摘　　要：目的通过网络药理学结合文献验证的方法,探讨妇科千金片/胶囊治疗盆腔炎性疾病后遗症(sequelae of pelvic inflammatory disease, SPID)的作用机制。方法基于TCMSP、TCMID等数据库及文献收集妇科千金片/胶囊的化学成分,筛选后获得活性成分及对应靶点;采用GeneGards数据库获得SPID相关靶点;采用STRING数据库对两者交集靶点进行蛋白互作分析;采用DAVID数据库进行基因功能和通路富集分析;采用Cytoscape软件构建"活性成分-靶点-通路"网络,结合文献进行评价。结果从妇科千金片/胶囊中筛选得到活性成分88个,对应靶点597个,与SPID交集靶点169个;黄芩素、小檗碱、木犀草素等77个化学成分可能为妇科千金片/胶囊的主要活性成分;雌激素受体1(estrogen receptor 1, ESR1)、前列腺素内过氧化物合酶2(prostaglandin endoperoxide synthase 2, PTGS2)、一氧化氮合酶2(nitric oxide synthase 2, NOS2)等80个靶点可能为妇科千金片/胶囊治疗SPID的关键靶点;磷脂酰肌醇-3-激酶/丝苏氨酸蛋白激酶(phosphateidylinositol 3 kinase/serine-threonine kinase, PI3K-Akt)、缺氧诱导因子1(hypoxia inducible factor-1, HIF-1)、肿瘤坏死因子(tumor necrosis factor, TNF)等9条信号通路可能为妇科千金片/胶囊发挥治疗作用的主要途径。结论妇科千金片/胶囊可通过多成分、多靶点协同治疗SPID,其作用机制可能与调控PI3K-Akt通路、改善血液循环及调节炎症、免疫反应和凋亡有关。Objective To investigate the effect of Fuke Qianjin Tablet/Capsule on sequelae of pelvic inflammatory disease(SPID) by network pharmacology combined with literature validation. Methods The chemical constituents of Fuke Qianjin Tablet/Capsule were collected based on TCMSP, TCMID and other databases and literatures, and the active constituents and corresponding targets were obtained after screening. GeneGards database was used to obtain SPID related targets. STRING database was used to analyze the protein interactions of the intersection targets. DAVID database was used for gene function and pathway enrichment analysis. Cytoscape software was used to construct an “active component-target-pathway” network, which was evaluated in conjunction with the literature. Results 88 active components were screened from Fuke Qianjin Tablet/Capsule, with 597 corresponding targets and 169 intersection targets with SPID. Wogonin, berberine, luteolin and other 77 chemical components may be the main active components of Fuke Qianjin Tablet/Capsule;the 80 targets such as estrogen receptor 1, prostaglandin endoperoxide synthase 2 and nitric oxide synthase 2 may be the key targets for the treatment of SPID by Fuke Qianjin Tablet/Capsule. Nine signaling pathways, including phosphateidylinositol 3 kinase/serine-threoninekinase, hypoxia inducible factor-1 and tumor necrosis factor may be the main pathways for the therapeutic effect of Fuke Qianjin Tablet/Capsule. Conclusion Fuke Qianjin Tablet/Capsule can treat SPID through multi-component and multi-target synergistic therapy, and its mechanism may be related to regulating PI3 K-Akt pathway, improving blood circulation, regulating inflammation, immune response and apoptosis.

关 键 词：妇科千金片 妇科千金胶囊 盆腔炎性疾病后遗症 靶点 网络药理学 

分 类 号：R285.5[医药卫生—中药学]