健脾柔肝息风汤对抽动障碍模型大鼠脑组织神经元中DARPP-32、TH含量的干预作用  

Effect of Jianpi Rougan Xifeng Decoction on the content of DARPP-32 and TH in the brain neurons of model rats with tic disorder

作　　者：梁芸 朱沁泉 张涤[2] LIANG Yun;ZHU Qinquan;ZHANG Di(Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha,Hunan 410007,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《湖南中医药大学学报》2022年第2期235-239,共5页Journal of Hunan University of Chinese Medicine

基　　金：湖南省教育厅项目(17A159)。

摘　　要：目的研究健脾柔肝息风汤对抽动障碍(TD)模型大鼠皮质-纹状体-丘脑-皮质回路中多巴胺和环磷酸腺苷调节的磷蛋白(DARPP-32)、酪氨酸羟化酶(TH)含量的影响及其作用机制。方法将40只SD大鼠随机分为空白组(10只),造模组(30只),造模成功后,将造模组随机分为模型组、氟哌啶醇组和健脾柔肝息风汤组,每组10只,腹腔注射给药7 d,采用HE染色观察大鼠大脑皮层各区形态结构和神经元细胞形态变化,采用免疫组织化学染色检测DARPP-32及TH的表达。结果与空白组比较,模型组运动行为评分、刻板行为评分均上升,差异有统计学意义(P<0.05)。与模型组比较,健脾柔肝息风汤组、氟哌啶醇组运动行为评分、刻板行为评分均下降,差异有统计学意义(P<0.05)。与空白组大鼠比较,模型组、氟哌啶醇组及健脾柔肝息风汤组大鼠的DARPP-32、TH水平降低(P<0.05);与模型组比较,氟哌啶醇组及健脾柔肝息风汤组DARPP-32、TH水平升高,其平均光密度差异有统计学意义(P<0.05);氟哌啶醇组与健脾柔肝息风汤组DARPP-32、TH平均光密度比较,差异无统计学意义(P>0.05)。结论健脾柔肝息风汤可升高TD模型大鼠脑组织中DARPP-32、TH的含量,从而调节多巴胺能神经系统功能平衡,减少大鼠抽动症状的产生。Objective To study the effect of Jianpi Rougan Xifeng Decoction on the content of dopamine and adenosine3’5’-monophosphate-regulated phospho-protein(DARPP-32) and tyrosine hydrogenase(TH) in the cortex striatum-thalamus-cortex loop of model rats with tic disorder, and to explore its mechanism. Methods 40 SD rats were randomly divided into blank group(n=10) and modeling group(n=30), after successful modeling, the modeling group was randomly divided into model group, haloperidol group and Jianpi Rougan Xifeng Decoction group, with 10 rats in each group. 7 days after intraperitoneal injection, HE staining was used to observe the morphological structure of each area of cerebral cortex and the morphological changes of neurons, and immunohistochemical staining was used to detect the expression of DARPP-32 and TH. Results Compared with blank group, motor behavior score and rigid behavior score of model group increased, and the differences were statistically significant(P <0.05).Compared with model group, motor behavior score and rigid behavior score of Jianpi Rougan Xifeng Decoction group and haloperidol group were decreased, and the differences were statistically significant(P<0.05). Compared with blank group, the levels of DARPP-32 and TH in model group, haloperidol group and Jianpi Rogan Xifeng Decoction group decreased(P <0.05).Compared with model group, the levels of DARPP-32 and TH in haloperidol group and Jianpi Rogan Xifeng Decoction group increased, and their average optical density was significantly different(P <0.05). And there was no significant difference in the average optical density of DARPP-32 and TH between the haloperidol group and the Jianpi Rougan Xifeng Decoction group(P>0.05). Conclusion Jianpi Rougan Xifeng Decoction can increase the content of DARPP-32 and TH in brain tissues of rats with tic disorder, thus regulating the functional balance of dopaminergic nervous system and reducing the occurrence of tic symptoms.

关 键 词：儿童抽动障碍 DARPP-32 酪氨酸羟化酶 健脾柔肝息风汤 大鼠 

分 类 号：R272.6[医药卫生—中医儿科学]