Parallel isolation of calicivirus and reovirus from lethal co-infected mink during a potential epidemic of farmed mink infections  

作　　者：Yuanyuan Guo William J.Liu Jingdong Song Kexin Zong Hao Lin Xin Li Shuting Huo Sai Liu Hongli Ran Hongmei Li Peipei Liu He Huang George F.Gao Guizhen Wu 

机构地区：[1]School of Pharmaceutical Sciences,Nanjing Tech University,No.30 South Puzhu Road,Nanjing 211816,China [2]NHC Key Laboratory of Biosafety,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China [3]School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou 325035,China [4]School of Ophthalmology and Optometry,Wenzhou Medical University,Wenzhou 325035,China [5]School of Food Science and Pharmaceutical Engineering,Nanjing Normal University,Wenyuan Road,Nanjing 210023,China [6]CAS Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences(CAS),Beijing 100101,China

出　　处：《Biosafety and Health》2021年第5期281-291,共11页生物安全与健康（英文）

基　　金：supported in part by the Major Special Projects for Infectious Disease Research of China(Grant 2018ZX10101002-005);the Research Units of Adaptive Evolution and Control of Emerging Viruses,Chinese Academy of Medical Sciences(Grant 2018RU009);the National Key Research and Development Program of China(Grant 2020YFC0840800);the Young Scientists Research Fund of Academician Yunde Hou.

摘　　要：Mink has been identified as an animal with susceptibility to SARS-CoV-2 and also as the only animal with evidence to transmit the virus back to humans.Thus,the surveillance of viruses among high-density farmed minks has a significant meaning for the control of zoonotic emerging diseases in humans.Within anal swabs of minks that died of unknown causes in a mink farm,mink calicivirus(MCV)and mammalian orthoreovirus(MRV)were detected and simultaneously observed within MDCK cell culture from the sample of the same lethal mink.The parallel isolation was successfully performed by utilizing cell lines from different host sources with distinct viral sensitivities,i.e.Mv.1.Lu and Vero-E6 and the two viruses were independently separated.The prevalence of the virus among the minks and its genomic characteristics were investigated through deep sequencing technology.Phylogenetic analysis of the viral genome showed a close relationship of the newly isolated MCV-GCCDC8-2020 with MCV strains belonging to the genus Vesivirus,but with unique mutations derived from the major structural protein(VP1).The reovirus MRV-GCCDC9-2020 isolated from the same mink belongs to serotype 3 mammalian orthoreovirus and genome analysis showed a potential reassortment derived from reoviruses in different species.This study provides a beneficial reference on viral co-infection within disease investigation in farmed minks and raises the concern for the virus surveillance among the high-density fed animal farms.

关 键 词：Mink virus CO-INFECTION Parallel isolation Evolutionary analyses EPIDEMIC 

分 类 号：S858.92[农业科学—临床兽医学]