基于Plackett-Burman和Box-Behnken设计优化超声诱导防风种子发芽方法  

Optimization of Ultrasonic Induced Germination of Saposhnikovia divaricate Seeds Based on Plackett-Burman and Box-Behnken Design

作　　者：李占君 刘运伟 王洪学 王岩[2] 房柱 兴成彬 LI Zhanjun;LIU Yunwei;WANG Hongxue;WANG Yan;FANG Zhu;XING Chengbin(Yichun Branch of Heilongjiang Academy of Forestry,Yichun 153000,China;Heilongjiang Academy of Forestry,Harbin 150081,China)

机构地区：[1]黑龙江省林业科学院伊春分院,黑龙江伊春153000 [2]黑龙江省林业科学院,哈尔滨150081

出　　处：《森林工程》2022年第1期76-85,共10页Forest Engineering

基　　金：中央引导地方科技发展专项(ZY19C11);黑龙江省伊春市青年基金科技项目(Q2020-1)。

摘　　要：为研究一种超声诱导促进防风种子发芽的方法,本文以超声时间、超声功率、水浴温度、超声频率以及浸种时间展开单因素影响实验。在单因素实验的基础上运用PBD(Plackett-Burman Design)进行关键因素的筛选,通过爬坡实验得出关键因素的中心点。结合BBD(Box-Behnken Design)响应面优化关键因素条件,最终得出最佳超声诱导防风种子发芽因素为:超声时间16 min、超声功率62 W、水浴温度35℃、超声频率40 Hz和浸种时间2 d,且预期发芽率和预期发生概率分别为68.21%和98.70%。该条件下防风种子发芽率为65.66%,其相对误差为3.74%。最佳超声诱导条件实验组与空白组相比,其发芽率、发芽势和发芽指数均优于空白组,且发芽率最终提升了29.71%。超声诱导处理与空白组种皮扫描电镜结果得知,种皮条纹裂隙阻塞程度与通透性会因超声过程中所产生的“空化效应”的作用得到较大程度的改善。研究表明适宜超声诱导处理对防风种子的萌发具有积极促进作用。In order to study a method of promoting seed germination of Saposhnikovia divaricate seed by ultrasonic induction,in this article,single factor experiments were carried out based on ultrasonic time,ultrasonic power,water bath temperature,ultrasonic frequency and seed soaking time.Based on the single factor experiment,Plackett-Burman Design(PBD)was used to screen the key factors,and the central point of the key factors was obtained through the climbing experiment.Optimize key factors and conditions in combination with Box-Behnken Design(BBD)response surface,the best ultrasonic-induced seed germination factors of the Saposhnikovia divaricata seed were:ultrasonic time 16 min,ultrasonic power 62 W,water bath temperature 35℃,ultrasonic frequency 40 Hz,seed soaking time 2 d,and the expected germination rate and expected occurrence probability were 68.21%and 98.70%,respectively.Under this condition,the germination rate of Saposhnikovia divaricate seed was 65.66%,the relative error was 3.74%.Compared with the CK group,the germination rate,germination potential and germination index of the experimental group were better than those of the CK group,and the germination rate was finally increased by 29.71%.The results of ultrasonic induction treatment and scanning electron microscope of CK seed coat showed that the blockage degree and permeability of seed coat stripe cracks could be greatly improved by"cavitation effect"produced in the process of ultrasound.Research showed that ultrasonic treatment had a positive effect on Saposhnikovia divaricate seed germination.

关 键 词：PBD BBD 超声诱导 防风种子 发芽率 种皮微观结构 

分 类 号：S723.131.1[农业科学—林木遗传育种] S718.3[农业科学—林学]