培养基种类与桑黄菌丝生长及黄酮含量的关系研究  

Effects of different media on mycelial growth and flavonoids content of Sanghuangporus vanini

作　　者：李秀娥 郑巧佳 张蕊 刘伟 吴其平 李维焕 程显好 Li Xiu’e;Zheng Qiaojia;Zhang Rui

机构地区：[1]鲁东大学农学院,山东烟台264025 [2]山东利农菌业有限公司,山东东营257442

出　　处：《食药用菌》2022年第1期57-61,共5页Edible and Medicinal Mushrooms

基　　金：山东省现代农业产业技术体系食用菌产业体系岗位专家项目[鲁农科技字(2016)18号];东营市第六批黄河三角洲学者项目[(东委(2016)84号)];烟台市重点研发项目(2019XDHZ092)。

摘　　要：为探究不同培养基对桑黄菌丝生长及黄酮含量的影响,通过在PDA、GPY、GPC、麦芽、麦麸以及胡萝卜培养基等6种基本培养基上接种桑黄进行发菌培养,并在不同生长时期对菌丝的生长速度、含水量、干湿重及黄酮含量进行分析比较,筛选最优培养基。结果:6种基本培养基均能被桑黄利用,其中PDA培养基上的菌丝生长速度最快;GPY和麦麸培养基的菌丝体黄酮含量整体较高,培养至第15天,胡萝卜培养基上的菌丝体黄酮含量增加显著,跃居第一,6种培养基上的菌丝体黄酮含量均随着培养时间的延长而增加。To explore the effects of different media on the mycelial growth and flavonoids content of Sanghuangporus vanini,the mycelia of S.vanini were cultured on six agar media: PDA medium,GPY medium,GPC medium,malt medium,wheat bran medium and carrot medium.The results showed that the six basic media could be used by S.vanini.Among them,wheat bran medium and GPY medium were the media with the highest flavonoids content,and PDA medium was the media with the fastest growth rate.The flavonoids conten of S.vanini on the six basic media increased with the extension of culture time.

关 键 词：桑黄 含水量 固体培养基 黄酮含量 

分 类 号：S567.39[农业科学—中草药栽培]