富砷湖滨湿地底泥优势细菌群落对风浪扰动的响应  

Environmental response to wind-wave disturbance of the dominant bacterial community in the sediment of arsenic-rich lakeside wetland

作　　者：呼唤 李丹蕾 王玉莹 刘云根[1,2] 王妍 HU Huan;LI Danlei;WANG Yuying;LIU Yungen;WANG Yan(School of Ecology and Environment,Southwest Forestry University,Kunming 650224,China;Key Laboratory of Ecological Environment Evolution and Pollution Control in Mountainous Rural Areas of Yunnan Province,Kunming 650224,China)

机构地区：[1]西南林业大学生态与环境学院,昆明650224 [2]云南省山地农村生态环境演变与污染治理重点实验室,昆明650224

出　　处：《应用与环境生物学报》2021年第6期1492-1499,共8页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(41761098,21767027);云南省基础研究计划项目(2019FB070)资助。

摘　　要：底泥微生物是影响湖滨湿地生态环境的重要因素.设置无风浪(无曝气)、中风浪(曝气量设为20 L/min)、大风浪(曝气量设为40 L/min)3种风浪扰动强度,利用16S rRNA高通量测序技术,分析风浪作用对富砷湖滨湿地底泥优势细菌群落的影响.结果显示:(1)不同风浪扰动下的5种优势纲群为γ-变形菌纲(Gammaproteobacteria)、厌氧绳菌纲(Anaerolineae)、δ-变形菌纲(Deltaproteobacteria)、拟杆菌纲(Bacteroidia)和梭状芽孢杆菌纲(Clostridia).γ-变形菌纲和δ-变形菌纲相对丰度的变化规律同变形菌纲完全一致,均表现为无风浪>中风浪>大风浪,拟杆菌纲和梭状芽孢杆菌纲的相对丰度在无风浪和中风浪条件下无显著差异,其相对丰度在大风浪条件下则显著高于无风浪和中风浪条件.(2)Spearman相关性分析表明,富砷湖滨湿地底泥氮、磷污染物形态影响的主要细菌纲群为α-变形菌纲(Alphaproteobacteria)、γ-变形菌纲、δ-变形菌纲、厌氧绳菌纲和拟杆菌纲.(3)冗余结果表明,不同风浪扰动影响下,湖滨湿地底泥中影响主要细菌纲群的环境因子为酸碱度(pH)、氧化还原电位(Eh)和溶解氧(DO),且菌群对环境因子的响应存在差异,如厌氧绳菌纲与pH均呈显著负相关,其相关性随风浪扰动强度的增大呈减小的趋势,拟杆菌纲则与DO呈负相关,其相关性随风浪扰动强度的增加逐渐降低.本研究明晰了富砷湖滨湿地底泥的优势细菌群落、主要环境影响因子及与风浪扰动的响应关系,结果可为自然环境下高原湖泊生态环境的修复提供新的思路和科学依据.Sediment microorganisms are recognized as one of the principal factors affecting the ecological environment of lakeside wetlands. However, the influence of wind-wave disturbance on the sediment microorganisms of arsenic-rich lakeside wetlands has received scant attention. This study investigated the topic by defining three wind-wave disturbance intensities: no wind-wave(no aeration), medium wind-wave(aeration rate of 20 L/min), and big wind-wave(aeration rate of 40 L/min). 16 S rRNA high-throughput sequencing technology was used to analyze the effect of wind waves on the dominant bacterial community in the sediment. The results indicated that:(1) Gammaproteobacteria, Anaerolinea, Deltaproteobacteria, Bacteroidia, and Clostridia were the five dominant classes of bacteria under different wind-wave disturbances. Gammaproteobacteria and Deltaproteobacteria belong to Proteobacteria, and the variation of their relative abundance was consistent with that of Proteobacteria, that is: no wind-wave > medium wind-wave > big wind-wave. The relative abundance of Bacteroidia and Clostridia was not significantly different under no wind-wave and medium wind-wave conditions, but was significantly higher under big wind-wave conditions.(2) Spearman correlation analysis showed that Alphaproteobacteria, Gammaproteobacteria, Deltaproteobacteria, Anaerolineae, and Bacteroidia were the main bacterial classes that affected the forms of nitrogen and phosphorus pollutants in the sediment of arsenic-rich lakeside wetlands.(3) Redundancy analysis(RDA) results showed that pH, Eh, and DO were the main environmental factors affecting the bacterial groups in the sediments of lakeside wetlands under different wind-wave disturbances, and that there were differences in response to environmental factors. For example, Anaerolineae was negatively correlated with pH, with a decrease in correlation accompanying an increase in wind-wave disturbance intensity;while Bacteroidia was negatively correlated with DO, with a decrease in correlation accompanying a

关 键 词：风浪扰动 氮形态 磷形态 环境响应 优势细菌 高原湖滨湿地 

分 类 号：X524[环境科学与工程—环境工程] X172