香蕉SUT基因家族的全基因组鉴定及其在不同低温胁迫下的表达  

Genome-wide identification and expression analysis of the SUT gene family under different low temperature stressors in banana(Musa nana Lour.)

作　　者：许小琼 张春渝 陈晓慧[1] 张梓浩[1] 林玉玲[1] 陈振光[1] 赖钟雄[1] XU Xiaoqiong;ZHANG Chunyu;CHEN Xiaohui;ZHANG Zihao;LIN Yuling;CHEN Zhenguang;LAI Zhongxiong(Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学园艺植物生物工程研究所,福州350002

出　　处：《应用与环境生物学报》2021年第6期1616-1625,共10页Chinese Journal of Applied and Environmental Biology

基　　金：国家重点研发计划项目(2019YFD1000901);国家现代农业产业技术体系(香蕉)专项资金(GARS-31-15);福建省高原学科建设经费(102/71201801101);福建农林大学科技创新专项基金项目(KF2015108、CXZX2013119、CXZX2017351、CXZX2017189、CXZX2018076)资助。

摘　　要：为探究香蕉蔗糖转运蛋白(sucrose/H+cotransporter或sucrose transporters,SUCs或SUTs)家族成员的分子特性及其在低温胁迫下的表达模式,采用生物信息学方法鉴定香蕉蔗糖转运蛋白基因家族成员,并进行蛋白结构域及特性、基因及蛋白序列组分、分子进化树、基因启动子顺式作用元件以及在不同低温处理下表达模式的分析.结果显示:香蕉A(Musa acuminata)基因组与香蕉B(Musa balbisiana)基因组中各存在6条SUT基因,分别命名为MaSUT1-6、MbSUT1-6,且均分为三大类:SUT2、SUT3、SUT4;根据基因序列组分分析可知,MaSUT的GC含量低于MbSUT的GC含量,且二者均低于水稻SUT基因的GC含量;蛋白互作显示,MaSUT、MbSUT成员可与多种蛋白存在互作关系;MaSUT家族成员启动子包含激素、光、非生物胁迫响应相关元件,且该家族成员均包含光响应元件;MaSUT、MbSUT基因成员在不同低温胁迫处理下存在表达显著下调(4℃)、表达显著上调(28℃、13℃、4℃)以及在各温度处理下均不表达的5种表达模式,而MaSUT1-2、MaSUT4、MbSUT1、MbSUT3-4在低温(13℃、4℃、0℃)处理下表达量均高于对照(28℃),MaSUT3和MbSUT2在低温处理下表达量则相对下降.本研究表明香蕉A、B基因组的SUT家族成员功能具有差异性,并且部分成员可能在香蕉响应低温胁迫的过程中发挥重要作用.This study aimed to explore the molecular characteristics of sucrose transporter(or sucrose/H+cotransporter, SUC, or SUT) family members in banana(Musa nana Lour.) and their expression patterns under low temperature stress. We used bioinformatics methods to identify members of the banana sucrose transporter gene family, and to analyze the protein domains and characteristics, gene and protein sequence components, molecular evolutionary trees, gene promoter cis-acting elements, and expression patterns under different low-temperature treatments. The results revealed 6 SUT genes in the M. acuminata and M. balbisiana genomes, named MaSUT1-6 and MbSUT1-6 respectively. The genes were then divided into 3 categories according to the analysis of gene sequence components, namely SUT2, SUT3, and SUT4. The GC content of MaSUT was lower than that of MbSUT, while the GC content of both MaSUT and MbSUT was lower than that of SUT genes in rice. Protein interaction prediction analysis revealed that MaSUT and MbSUT members could interact with a variety of proteins, and MaSUT family member promoters contained hormone-, light-, and abiotic stress response-related elements, and all family members contained light response elements. MaSUT and MbSUT gene members had five different expression patterns, namely a significant down-regulation(at 4 ℃);a significant up-regulation(at 28 ℃, 13 ℃, and 4 ℃);no expression under each temperature treatment;higher expression levels of MaSUT1-2, MaSUT4, MbSUT1, and MbSUT3-4 than those under low temperature(at 13 ℃,4 ℃, and 0 ℃) treatment;and lastly, in stark contrast, at 28 ℃, the expression levels of MaSUT3 and MbSUT2 were relatively decreased under the low-temperature treatment. This study shows that the functions of SUT family members in M. acuminata and M. balbisiana genomes are different, and some members might play an important role in banana responses to low-temperature stress.

关 键 词：香蕉 蔗糖转运蛋白 基因家族 全基因组鉴定 

分 类 号：S668.1[农业科学—果树学]