载玻片的亲、疏水性对玉米大斑病菌分生孢子萌发与芽管发育的影响  

Effects of Hydrophilicity and Hydrophobicity of Slides on Conidia Germination and Germ Tube Development of Setosphearia turcica

作　　者：胡净净 刘子怡 黄晓晗 高文强 曹志艳[2] 周丽宏 董金皋[1,2] HU Jing-Jing;LIU Zi-Yi;HUANG Xiao-Han;GAO Wen-Qiang;CAO Zhi-Yan;ZHOU Li-Hong;DONG Jin-Gao(College of Life Science,Hebei Agricultural University,Baoding 071000,China;College of Plant Protection,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北农业大学生命科学学院,保定071000 [2]河北农业大学植物保护学院,保定071000

出　　处：《农业生物技术学报》2022年第1期106-113,共8页Journal of Agricultural Biotechnology

基　　金：河北省引进留学人员资助项目(C20200338);现代农业产业技术体系专项资金(CARS-02)。

摘　　要：玉米大斑病(northern corn leaf blight,NCLB)是发生在玉米(Zea mays)叶部、由大斑病凸脐蠕孢菌(Setosphaeria turcica)(俗称大斑病菌)引起的真菌性病害。玉米大斑病菌侵染玉米叶片时首先感知叶片的亲、疏水性及硬度等物理性质。为研究叶片表面亲、疏水性的差异对玉米大斑病菌的影响,本研究在不同亲、疏水载玻片上培养了玉米大斑病菌的分生孢子,观察了不同时间内孢子的萌发率、附着胞形成率及糖原和脂滴分布的差异,并利用扫描电子显微镜观测了抗、感品种玉米叶片表面的微观结构差异。结果显示,在疏水载玻片上分生孢子的萌发率、附着胞产生率均高于在亲水载玻片上;分生孢子萌发产生的芽管内脂滴颗粒大小分布不同,在亲水载玻片上脂滴颗粒明显大于疏水载玻片上的脂滴颗粒;芽管内的糖原分布在亲、疏水载玻片上未观察到显著差异。抗、感玉米大斑病品种叶片的微观结构观察显示,抗病品种’郑单958’和感病品种’先玉335’叶片表面微观结构明显不同,’郑单958’叶片表面蜡质层更为粗糙。上述结果表明,亲、疏水性影响玉米大斑病菌分生孢子的萌发、附着胞形态及芽管内有机物质的累积。研究结果为玉米抗感品种的选育提供数据支持。Northern corn leaf blight(NCLB)occurrs on maize(Zea mays)leaf and is a foliar disease caused by Setosphaeria turcica.The physical properties of maize leaf,such as hardness,hydrophilicity,and hydrophobicity,can be perceived by S.turcica firstly when it infects maize leaves.The conidia germination rate,appressorium formation rate,lipid droplets,and glycogen distribution were observed.The results showed that the rate of conidia germination and appressorium formation on the hydrophobic substrate were higher than those on the hydrophilic substrate.The diameter of lipid droplets in germ tubes cultured on the hydrophilic substrate was larger than those cultured on the hydrophobic substrate.The distribution of glycogen in germ tubes was not significantly different.The surface morphology of the susceptible(’Xianyu 335’)and resistant(’Zhengdan 958’)variety to S.turcica was significantly different.The epicuticular wax layer of’Zhangdan 958’leaf was rougher than that of’Xianyu 335’leaf.These results indicated that the hydrophilicity of glass slide affects the development of the S.turcica conidia and provided data support for the breeding of maize.

关 键 词：玉米大斑病菌 亲、疏水性 糖原 脂肪 

分 类 号：S435.131.4[农业科学—农业昆虫与害虫防治]