不同地区多花黄精的ITS序列分析及近缘种聚类分析  

ITS Sequence Analysis and Cluster Analysis of Polygonatum cyrtonema Hua in Different Regions and Its Related Species

作　　者：宋荣 宋静爽[3] 欧立军 严蓓 周佳民 朱校奇[2] 易自立[1] Song Rong;Song Jingshuang;Ou Lijun;Yan Bei;Zhou Jiamin;Zhu Xiaoqi;Yi Zili(College of Bioscience and Biotechnogy,Hunan Agricultural University,Changsha,410128;Institute of Agricultural Environment and Ecology,Hunan Academy of Agricultural Sciences,Changsha,410125;Longping College Graduate School,Hunan University,Changsha,410125;College of Horticulture,Hunan Agricultural University,Changsha,410128;College of Plant Protection,Hunan Agricultural University,Changsha,410128)

机构地区：[1]湖南农业大学生物科学技术学院,长沙410128 [2]湖南省农业科学院农业环境生态研究所,长沙410125 [3]湖南大学研究生院隆平分院,长沙410125 [4]湖南农业大学园艺学院,长沙410128 [5]湖南农业大学植物保护学院,长沙410128

出　　处：《分子植物育种》2022年第1期218-224,共7页Molecular Plant Breeding

基　　金：湖南省现代农业中药材产业技术体系建设项目(湘财农指[2019]47号);国家中药材产业技术体系建设专项(CARS-21);湖南省农业科技创新项目(2018ZD05-2);南省农科院科技创新探索性基础研究项目(2017JC58)共同资助。

摘　　要：为建立快速鉴定多花黄精的方法及确定其亲缘关系,本研究采用DNA条形码分析的方法,通过对28个多花黄精材料的核糖体DNA内转录间隔区(ITS)片段进行扩增获得ITS序列,进行多重比对,并构建多花黄精及近缘种的系统发育分析树。结果表明:本研究共获得两种多花黄精ITS的单倍型,仅发生一个位点的突变,说明多花黄精种内保守性高,遗传稳定,并可作为多花黄精DNA条形码物种鉴定的对照序列;另外,其与穇属(Eleusine)中穇子(Eleusine coracana)物种亲缘关系最近。本研究为快速鉴定多花黄精提供了新方法,并为多花黄精的后续研究提供了依据。In order to establish a rapid method to distinguish Polygonatum cyrtonema Hua and identify their genetic relationships,the DNA barcode analysis method was used to analyze the sequence by amplifying the ribosomal DNA internal transcribed spacer(ITS)fragment of 28 Polygonatum cyrtonema Hua and conducting the multiple sequence alignment in this research.And then,we constructed the phylogenetic analysis tree of Polygonatum cyrtonema Hua and the relative species.The results showed that the Polygonatum had high conservation and genetic stability,which can be used as the control sequence for species identification of Polygonatum DNA bar code.We have obtained only two haplotypes of Polygonatum,in which a mutation site was discovered.In addition,we have noticed that the Polygonatum is closely related to Eleusine coracana species.Therefore,this study will provide a new method for rapidly distinguishing Polygonatum cyrtonema Hua and a basis for the follow-up study of Polygonatum cyrtonema Hua.

关 键 词：多花黄精 ITS 分子鉴定 聚类分析 

分 类 号：S567.239[农业科学—中草药栽培]