草原龙胆‘阿琳娜’高效再生体系的建立  

Establishment of Efficient Regeneration System for Eustoma grandiflorum’Arena’

作　　者：王坤 张春燕 殷倩 汪昱乔 王琴[1,2] 胡惠蓉 Wang Kun;Zhang Chunyan;Yin Qian;Wang Yuqiao;Wang Qin;Hu Huirong(Key Laboratory of Horticultural Plant Biology,Ministry of Education,Key Laboratory of Urban Agriculture in Central China,Ministry of Agriculture,College of Horticulture&Forestry Sciences,Huazhong Agricultural University,Wuhan,430070;College of Civil Engineering and Environment,Hubei University of Technology,Wuhan,430068)

机构地区：[1]华中农业大学园艺林学学院,农业部华中都市农业重点实验室,园艺植物生物学教育部重点实验室,武汉430070 [2]湖北工业大学土木建筑与环境学院,武汉430068

出　　处：《分子植物育种》2022年第1期239-245,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31972988);国家级大学生创新创业训练计划项目(201710504033)共同资助。

摘　　要：草原龙胆(Eustoma grandiflorum)花色典雅、花型丰富,是全球最受欢迎的切花之一。本研究以草原龙胆‘阿琳娜’叶片为外植体,研究了6-BA、NAA、GA_(3)和IBA不同组合对其出芽、壮苗、增殖、生根的影响。结果表明:最佳不定芽诱导培养基为MS+6-BA 0.5 mg/L+NAA 0.05 mg/L,不定芽诱导率达90.0%,平均出芽数为4.52个;MS培养基可实现壮苗;最佳增殖培养基为MS+6-BA 0.2 mg/L+IBA 0.2 mg/L+GA_(3)0.3 mg/L,增殖系数为6.41;培养基1/2MS+IBA 0.5 mg/L有利于生根。初步建立了草原龙胆‘阿琳娜’高效再生体系,为草原龙胆优良品种快速繁殖及基因工程研究提供了依据。Lisianthus(Eustoma grandiflorum)is one of the most popular cut flowers in the world,with elegant colors and rich flower patterns.In this study,leaves of lisianthus’Arena’were used as explants to study the effects of different combinations of 6-BA,NAA,GA_(3)and IBA on budding,strong seedling,proliferation and rooting.Results showed that the optimum adventitious bud induction medium was MS+6-BA 0.5 mg/L+NAA 0.05 mg/L,the adventitious bud induction rate was 90.0%,and the average number of buds was 4.52.The MS medium could be used for strong seedling.The optimum proliferation medium was MS+6-BA 0.2 mg/L+IBA 0.2 mg/L+GA_(3)0.3 mg/L,and the proliferation coefficient was 6.41.The medium with 1/2MS+IBA 0.5 mg/L was good for rooting.Thus,a high-efficiency regeneration system of lisianthus’Arena’was preliminarily established,which provided a basis for the rapid propagation and genetic engineering breeding of elite varieties of lisianthus.

关 键 词：草原龙胆‘阿琳娜’ 叶片 再生体系 

分 类 号：S682.19[农业科学—观赏园艺]