人血白蛋白产品对人干细胞培养的影响  

Effects of human albumin products on human stem cell culture

作　　者：程露 徐俊 CHENG Lu;XU Jun(Shanghai RAAS Blood Products Co.Ltd,Shanghai 201401,China)

机构地区：[1]上海莱士血液制品股份有限公司,上海201401

出　　处：《中国输血杂志》2021年第12期1385-1387,共3页Chinese Journal of Blood Transfusion

摘　　要：目的比较国内不同厂家的人血白蛋白产品对人干细胞培养的影响。方法在无血清培养基中加入10种不同厂家的人血白蛋白产品对人CD34+干细胞进行培养,研究细胞在15 d内的扩增情况。通过液质联用技术分析不同来源的人血白蛋白后修饰的情况。结果于15 d内,在无血清培养基中加入血源性人血白蛋白产品可使干细胞扩增最高达(20.6±5.7)倍,而加入重组人血白蛋白则无明显促进扩增效果;液质分析发现血源性的与重组的人血白蛋白产品后修饰有明显差异。结论不同厂家的人血白蛋白对促进干细胞的扩增有明显的差异,血源性的人血白蛋白对干细胞的培养效果优于重组人血白蛋白。血源性的与重组的人血白蛋白在后修饰上的差异是否与干细胞扩增存在相关性,有待进一步研究。Objective To compare the effects of human albumin products from different domestic manufacturers on human stem cell culture.Methods Human CD34+ cells were cultured by supplementing human albumin from different manufacturers to serum-free medium, and the expansion ratios of cells within 15 days were counted. Post translational modifications of human albumin products were studied by LC-MS.Results Supplementing plasma-derived human albumin(pd-alb) to serum-free medium, the expansion ratios of cells could reach up to 20.6±5.7, while the recombinant human albumin(rhAlb) resulted in no significant expansion within 15 days. LC-MS showed significant differences in post-translational modifications from different human albumin products.Conclusion Different human albumin products showed significantly different effects in the expansion of stem cells due to different sources, processes and stabilizers. Pd-alb products were better for stem cell culture than rhAlb products. There are significant differences between pd-albs and rhAlbs in post-translational modification, but whether these differences are related to stem cell expansion remains to be studied.

关 键 词：人血白蛋白 重组白蛋白 干细胞 翻译后修饰 

分 类 号：Q512.1[生物学—生物化学]