Dietary intake of bamboo vinegar and charcoal powder(BVC)enhances resistance of African catfi sh Clarias gariepinus to bacterial pathogen  

作　　者：Kwangjin JU Moyngsuk KIL Sanghyok RI Tongchol KIM Lining ZHANG Maocang YAN Guangxu LIU 

机构地区：[1]College of Animal Sciences,Zhejiang University,Hangzhou 310058,China [2]College of Aquaculture,Wonsan Fisheries University,Wonsan 999093,DPR Korea [3]College of Life Science,Kim Hyong Jik University of Education,Pyongyang 99903,DPR Korea [4]Zhejiang Mariculture Research Institute,Wenzhou 325005,China

出　　处：《Journal of Oceanology and Limnology》2022年第1期336-346,共11页海洋湖沼学报（英文）

基　　金：Supported by the National Key R&D Program of China(No.2018YFD0900603);the National Natural Science Foundation of China(No.31672634);the Open Project of Key Laboratory of Environmental Biotechnology,CAS(No.kf2018005);the Science and Technology Project of Wenzhou(No.2019ZX002-02)。

摘　　要：Although the immunomodulatory activity of bamboo vinegar and charcoal powder(BVC)has been increasingly documented in domestic animals,the use of BVC as immunostimulant in fi sh aquaculture awaits verifi cation.In the present study,the immunostimulatory impacts of dietary BVC intake were investigated in an important aquaculture fi sh species,the African catfi sh Clarias gariepinus.Results show that the pathogen resistance of African catfi sh was signifi cantly improved by dietary BVC intake at the tested levels(0.5%-2%),as indicated by higher survival rates(approximately 1.52-1.85 times that without BVC supplementation)upon intraperitoneally injection of bacterial pathogen Aeromonas hydrophila.In addition,both the count and phagocytic activity of blood cells were signifi cantly up-regulated by a 45-day dietary supplementation of BVC.Moreover,compared to that of the control,fi sh individuals fed BVC containing diets exhibited signifi cant higher activities of superoxide dismutase(SOD),myeloperoxidase(MPO),and lysozyme(LYZ).The content of immunoglobulin M(IgM)in serum of African catfi sh was also induced by dietary BVC supplementation.Furthermore,the expression levels of interleukin-8(IL-8),toll-like receptor 1 and 5(TLR1 and TLR5),myeloid diff erentiation factor 88(MyD88),and nuclear factor kappa B(NFκB)in the head kidney were signifi cantly up-regulated.Our fi ndings indicate BVC could be an eff ective immunostimulant in teleost species,which may enhance fi sh immunity through improving hematic parameters,activating bioactive humoral molecules,and up-regulating immune related molecular pathways.

关 键 词：bamboo vinegar and charcoal powder(BVC) African catfi sh IMMUNOSTIMULANT pathogen resistance 

分 类 号：S943[农业科学—水产养殖]