Comparative transcriptomic analysis of Macro brachium nippon ense in response to Aerom onas veronii or Staphylococcus au reus infection  

作　　者：Chong HAN Zhipeng ZHANG Qiang LI Qinghua CHEN Jianrong HUANG 

机构地区：[1]State Key Laboratory of Biocontrol,Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals,School of life Sciences,Sun Yat-Sen University,Guangzhou 510275,China [2]Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),Zhuhai 519082,China [3]School of life Sciences,Guangzhou University,Guangzhou 510006,China [4]South China Institute of Environmental Science,MEE,Guangzhou 510610,China

出　　处：《Journal of Oceanology and Limnology》2022年第1期347-359,共13页海洋湖沼学报（英文）

基　　金：Supported by the Research on the Diversity of Freshwater Fish,Shrimp and Crab in Macao(No.20183300042411016);the Guangdong Special Environmental Fund(Nos.PM-zx555-202107-208,PM-zx555-202106-195)。

摘　　要：Macrobrachium nipponense is an economically important freshwater prawn that is often threatened by many aquatic pathogens.In this study,comparative transcriptomic analysis was fi rstly used to explore the transcriptional response of M.nipponense to Aeromonas veronii or Staphylococcus aureus stimulation.A total of 400.19 million clean reads were obtained and assembled into 56944 unigenes with an average length of 1253 bp.A total of 1857 diff erentially expressed genes were found after A.veronii infection,including 677 genes that were up-regulated and 1180 genes that were down-regulated,while 1061 signifi cant diff erentially expressed genes were identifi ed after S.aureus infection,including 390 up-regulated and 671 down-regulated genes.Many immune-related genes including Spaetzle,prophenoloxidase activating factor,C-type lectin,anti-lipopolysaccharide factor,and inhibitor of apoptosis 2 protein were commonly up-regulated after A.veronii or S.aureus infection.This study will enrich our understanding of the immune response to gram-positive and gram-negative bacteria infection in crustaceans.

关 键 词：Macrobrachium nipponense differentially expressed genes Aeromonas veronii Staphylococcus aureus immune response 

分 类 号：S945.4[农业科学—水产养殖]