Effects of gibberellin priming on seedling emergence and transcripts involved in mesocotyl elongation in rice under deep direct-seeding conditions  

作　　者：Ya WANG Yuetao WANG Ruifang YANG Fuhua WANG Jing FU Wenbo YANG Tao BAI Shengxuan WANG Haiqing YIN 

机构地区：[1]Cereal Crops Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China [2]Crop Breeding and Cultivation Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2021年第12期1002-1021,共20页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：supported by the National Key Research and Development Program of China(No.2016YFD0100101-19);the Rice Industry Technology System of Henan Province(No.S2012-04);the Independent Innovation Fund Program of Henan Academy of Agricultural Sciences(No.2020ZC07)。

摘　　要：Mesocotyl elongation is a key trait influencing seedling emergence and establishment in direct-seeding rice cultivation.The phytohormone gibberellin(GA)has positive effects on mesocotyl elongation in rice.However,the physiological and molecular basis underlying the regulation of mesocotyl elongation mediated by GA priming under deep-sowing conditions remains largely unclear.In the present study,we performed a physiological and comprehensive transcriptomic analysis of the function of GA priming in mesocotyl elongation and seedling emergence using a direct-seeding japonica rice cultivar ZH10 at a5-cm sowing depth.Physiological experiments indicated that GA priming significantly improved rice seedling emergence by increasing the activity of starch-metabolizing enzymes and compatible solute content to supply the energy essential for subsequent development.Transcriptomic analysis revealed 7074 differentially expressed genes(false discovery rate of<0.05,|log2(fold change)|of≥1)after GA priming.Furthermore,gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analyses revealed that genes associated with transcriptional regulation,plant hormone biosynthesis or signaling,and starch and sucrose metabolism were critical for GA-mediated promotion of rice mesocotyl elongation.Further analyses showed that the expression of the transcription factor(TF)genes(v-myb avian myeloblastosis viral oncogene homolog(MYB)alternative splicing 1(MYBAS1),phytochrome-interacting factors 1(PIF1),Oryza sativa teosinte branched 1/cycloidea/proliferating cell factor 5(Os TCP5),slender 1(SLN1),and mini zinc finger 1(MIF1)),plant hormone biosynthesis or signaling genes(brassinazole-resistant 1(BZR1),ent-kaurenoic acid oxidase-like(KAO),GRETCHEN HAGEN 3.2(GH3.2),and small auxin up RNA 36(SAUR36)),and starch and sucrose metabolism genes(α-amylases(AMY2 A and AMY1.4))was highly correlated with the mesocotyl elongation and deep-sowing tolerance response.These results enhance our understanding of how nutrient metabolism-related

关 键 词：MESOCOTYL Rice(Oryza sativa L.) Direct-seeding GIBBERELLIN Deep sowing Transcriptomic analysis 

分 类 号：S511[农业科学—作物学]