高良姜PAL基因的序列和表达模式分析  

Analysis on Sequence and Expression Pattern of PAL Gene from Alpinia officinarum Hance

作　　者：黄琼林 HUANG Qiong-lin(School of Basic Medicine,Guangdong Medical University,Zhanjiang 524023,China)

机构地区：[1]广东医科大学基础医学院,广东湛江524023

出　　处：《现代中药研究与实践》2021年第6期13-16,共4页Research and Practice on Chinese Medicines

基　　金：广东省中医药局中医药科研项目(20211224)。

摘　　要：目的分析高良姜黄酮类生物合成第一步反应的关键酶——苯丙氨酸解氨酶(Phenylalanine ammonia-lyase,PAL)编码基因的序列特征,并明确该基因在高良姜中的表达模式。方法在高良姜转录组测序数据中挖掘PAL基因,采用生物信息学方法分析其编码蛋白的理化特征、组成、结构和细胞亚定位,并采用实时定量PCR分析该基因在高良姜不同组织中表达差异。结果获得的AoPAL cDNA序列长度为2261 bp,包含2130 bp编码区,编码710个氨基酸。AoPAL含有苯丙氨酸解氨酶保守功能域,归属苯丙氨酸解氨酶超级家族,且为不含跨膜结构域、信号肽和转运肽的亲水性混合结构型蛋白质。AoPAL表达在高良姜不同组织中呈现特异性,相对表达量为叶<茎<根茎。结论为AoPAL的功能鉴定奠定了研究基础,为阐明高良姜黄酮类物质生物合成途径研究提供理论参考。Objective To analyze the sequence feature and expression difference of PAL gene in traditional Chinese medicine Alpinia officinarum Hance.Methods PAL gene is annotated from the transcriptome of A.officinarum and then analyzed using bioinformatics approaches and the expression of PAL gene is analyzed using real-time quantitative PCR.Results Acquired AoPAL gene contained 2130 bp coding region and encoded 710 amino acids.AoPAL,the coding protein of AoPAL contained a phenylalanine ammonia-lyase functional domain,and no transmembrane region,signal peptide and transport peptide.AoPAL is a hydrophilic,mix-structural and stable protein.The expression of AoPAL exhibited tissue-specificity in A.officinarum and increased from leaf,stem to rhizome in turn.Conclusion It provides a firm foundation for the functional identification of AoPAL and flavones biosynthesis regulation in A.officinarum.

关 键 词：苯丙氨酸解氨酶 高良姜 CDNA序列 表达差异 生物信息学 

分 类 号：R282.5[医药卫生—中药学]