基于试验设计方法优化香薷挥发油的提取工艺  

Extraction Condition Optimization for Volatile Oil from Moslae Herba Based on Design of Experiment Methodology

作　　者：陈海鹰[1] 左晶 左小明[1] 朱珊梅[1] CHEN Hai-ying;ZUO Jing;ZUO Xiao-ming;ZHU Shan-mei(Department of Pharmacy,The Third People’s Hospital of Changzhou,Changzhou 213000,China;Department of Emergency,The Third People’s Hospital of Changzhou,Changzhou 213000,China)

机构地区：[1]常州市第三人民医院药事科,江苏常州213000 [2]常州市第三人民医院急诊科,江苏常州213000

出　　处：《现代中药研究与实践》2021年第6期64-69,共6页Research and Practice on Chinese Medicines

基　　金：常州四药临床药学科研基金指导性项目(2014280)。

摘　　要：目的优化香薷挥发油的水蒸气蒸馏法提取工艺。方法根据单因素试验结果,以挥发油提取率为指标,采用Plackett-Burman试验设计筛选出香薷挥发油提取过程的主要影响因素。在此基础上,利用Box-Behnken试验设计三因素三水平试验,运用Minitab软件对试验数据进行多元线性回归和二项式方程拟合,应用响应面分析法优化最佳提取工艺参数。结果最佳提取工艺参数:电热套温度为245.1℃,超声时间为29.70 min,液料比为12.87 mL/g。结论Plackett-Burman试验设计联合Box-Behnken设计-响应面分析法优化的香薷挥发油提取工艺稳定可行。Objective To optimize extraction process of volatile oil from Moslae Herba by steam distillation.Methods According to the results of single factor experiments,with extraction yield used as the evaluation index,Plackett-Burman design is adopt to screen the main factors which influenced the extraction process of Moslae Herba volatile oil.Three levels of the three factors are selected by Box-Behnken design.Minitab software is applied to analyze the experimental data and establish the multiple linear regression and binomial fitting.Response surface analysis is utilized to optimize the extraction process parameters.Results The best extraction process parameters are as follows:the temperature of heating mantle is 245.1℃,the ultrasonic time is 29.70 min,the liquid solid ratio is 12.87 mL/g.Conclusion The extraction process optimized by Plackett-Burman combined with Box-Behnken design-response surface analysis are stable and feasible.

关 键 词：香薷挥发油 Plackett-Burman试验设计 BOX-BEHNKEN试验设计 响应面分析法 

分 类 号：R283.3[医药卫生—中药学]