雌激素受体基因调控团头鲂生长和性腺发育的初步研究  

Expression and regulation of estrogen receptor genes on growth and sexual maturation in blunt snout bream,Megalobrama amblycephala

作　　者：刘莉芳 罗丽飞 陈宇龙 高泽霞[1,2,3] LIU Lifang;LUO Lifei;CHEN Yulong;GAO Zexia(Key Laboratory of Freshwater Animal Breeding,Ministry of Agriculture and Rural Affairs/Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction of Ministry of Education/Engineering Technology Research Center for Fish Breeding and Culture in Hubei Province,College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China;Hubei Hongshan Laboratory,Wuhan 430070,China;Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education,Wuhan 430070,China)

机构地区：[1]华中农业大学水产学院/农业农村部淡水生物繁育重点实验室/农业动物遗传育种与繁殖教育部重点实验室/湖北省名优鱼育种与健康养殖工程技术研究中心,武汉430070 [2]湖北洪山实验室,武汉430070 [3]长江经济带大宗水生生物产业绿色发展教育部工程研究中心,武汉430070

出　　处：《华中农业大学学报》2022年第1期179-185,共7页Journal of Huazhong Agricultural University

基　　金：国家重点研发计划“蓝色粮仓科技创新”专项(2018YFD0900205);国家自然科学基金项目(31872559);国家大宗淡水鱼类产业技术体系建设项目(CARS-45-08)。

摘　　要：通过基因克隆获得了团头鲂3种雌激素受体(estrogen receptor,ER)基因的CDS(coding sequence)序列:ERα(1904 bp)、ERβ1(1842 bp)和ERβ2(2609 bp),比较不同物种间的ERs基因的同源性,结果显示团头鲂ERs与鲤、金鱼和斑马鱼具有较高的同源性。为分析ERs对团头鲂性腺与生长发育的调控作用,分别以团头鲂不同年龄阶段的生长快慢群体为研究对象,比较分析ERs在不同年龄阶段生长快慢个体肌肉和性腺组织中的表达量。结果显示,在1龄到3龄团头鲂肌肉中,ERα和ERβ1在生长快组(FG)中的表达量整体高于生长慢组(SG)(P<0.05),并且ERα在1龄FG和SG团头鲂中的表达量差异比2龄和3龄团头鲂更为明显(P<0.05)。ERβ2在FG和SG团头鲂中的表达量差异与ERα和ERβ1相反(P<0.05),且在3龄雄性团头鲂中,ERβ2在FG组中的表达量极显著高于SG组。在1龄和2龄雌性团头鲂中,雌激素受体基因ERα、ERβ1和ERβ2在FG组的表达量显著高于SG组。在雄性团头鲂中,ERα在1龄FG组的性腺中的表达量高于SG组;1龄和2龄雄性团头鲂性腺中,ERβ2在FG组的表达量显著低于SG组,但在3龄雄性性腺中,ERβ2在FG组的表达量显著高于SG组。以上研究结果表明,ERs在一定程度上可促进鱼类生长和性腺发育,ERα、ERβ1和ERβ2可能组成一个复杂的调控体系,彼此相互影响,并共同调节机体生长和性腺发育。Estrogen is an essential steroid hormone that promotes fish gonad development and maintains reproductive function,contributing to regulate energy metabolism and growth development.Estrogen receptors(ERs)are the key receptor that mediate estrogen regulation in multiple tissues and organs.Therefore,ERs may be related to fish growth and gonadal development.In this study,we cloned the coding sequences(CDS)of estrogen receptor genes in blunt snout bream(Megalobrama amblycephala),including ERα(1904 bp),ERβ1(1842 bp)and ERβ2(2609 bp).Through comparing the homology of ERs among different species,we found that the ERs of M.amblycephala share high similarity with carp,goldfish and zebrafish.To analyze the relationship between ERs and gonad and growth,we collected one-year,two-year,and three-year old M.amblycephala,and selected individuals with fast growth(FG)and slow growth(SG)at each age stage.The expression of ERs in the gonad and muscles of M.amblycephala at different ages was analyzed.The results showed that in the early stage of gonadal development,ERa tended to play an important role in growth rather than gonad development.At the metaphase of gonadal development,ERαhad an effect on gonad development,and ERβ1 replaced the effect of ERa in growth.After gonadal maturation,the expression of ERαand ERβ1 in the FG group was significantly higher than the SG group,but the expression difference of ERβ1 between FG and SG groups was only shown in males,while ERαaffected both males and females.In addition,the expression difference of ERβ2 between FG and SG groups was contrary to that of ERαand ERβ1,which inhibit growth in the early and middle stages of gonadal development.Taken together,ERs could promote the gonads and growth of fish to a certain extent.Meantime,ERα,ERβ1 and ERβ2 might form a complex regulatory system to influence with each other and jointly regulate body growth and gonadal development.

关 键 词：团头鲂 雌激素受体基因 生长 性腺发育 基因表达 

分 类 号：Q78[生物学—分子生物学] Q959.468