嗅闻佛手柑精油对阿尔兹海默症小鼠的神经保护作用研究  

Neuroprotective effects of smelling bergamot essential oil on mice with Alzheimer’s disease

作　　者：胡慧艳 任婧楠 李晓 潘思轶 范刚 HU Huiyan;REN Jingnan;LI Xiao;PAN Siyi;FAN Gang(College of Food Science and Technology/Ministry of Education Key Laboratory of Environment Correlative Dietology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学食品科学技术学院/环境食品学教育部重点实验室,武汉430070

出　　处：《华中农业大学学报》2022年第1期229-237,共9页Journal of Huazhong Agricultural University

基　　金：中央高校基本科研业务费专项(2662020SPPY002)。

摘　　要：为研究嗅闻佛手柑精油(BEO)不同时间对D-半乳糖(D-gal)和三氯化铝(AlCl_(3))诱导的阿尔兹海默症小鼠的神经保护作用,选用健康的雄性昆明小鼠通过接受D-gal(120 mg/kg,腹腔注射)和AlCl_(3)(10 mg/kg,腹腔注射),持续49 d以建立阿尔兹海默症实验模型。第29天,阳性组喂服盐酸多奈哌齐,嗅闻组吸入BEO 1 h或2 h,持续21 d。治疗后评估了小鼠行为和大脑生物化学的变化。行为学研究结果显示,D-gal和AlCl_(3)明显减少了自发的交替和运动活动,表明行为和认知能力的损害。生化研究结果显示,D-gal和AlCl_(3)明显增加了氧化应激的程度、炎症因子的数量和乙酰胆碱酯酶(acetylcholinesterase,AchE)的含量。而2 h嗅闻组的小鼠的认知行为障碍和生化指标的改变得到了明显的改善。研究结果表明吸入BEO 21 d,尤其是2 h嗅闻组可以减轻D-gal和AlCl_(3)引起的认知行为障碍和生化损伤。Bergamot essential oil(BEO)is a very common citrus essential oil,which is widely used in the cosmetics and food industries.At the same time,it has a good effect on improving the mood of mice and anti-inflammatory and analgesic as well.This article evaluated the neuroprotective effects of inhaling BEO for various times on mice with Alzheimer’s disease induced by D-galactose(D-gal)and aluminum trichloride(AlCl_(3)).Healthy male Kunming mice received D-gal(120 mg/kg,intraperitoneal injection)and AlCl_(3)(10 mg/kg,intraperitoneal injection)for 49 days were used to establish an Alzheimer’s disease model.On the 29th day,the positive group received donepezil hydrochloride(oral),the sniffing group inhaled BEO for 1 h or 2 h for 21 d.After treatment,the cognitive behavior of the mice was evaluated by Y-maze and open field test(OFT),and the changes in brain biochemistry were detected by enzyme-linked immunosorbent assay(ELISA).The results of behavioral studies showed that D-gal and AlCl_(3) significantly reduced spontaneous alternation and motor activities,indicating that the cognitive ability of mice was impaired.The results of biochemical studies showed that D-gal and AlCl_(3) significantly increased the degree of oxidation stress,the number of inflammatory factors and the content of acetylcholinesterase(AchE).However,the cognitive behavioral impairment and the changes of biochemical indexes of the mice in the 2 h sniffing group were significantly improved.It is indicated that inhalation of BEO for 21 d,especially in the 2 h sniffing group,can significantly reduce the behavioral disorders and biochemical damage caused by D-gal and AlCl_(3).

关 键 词：阿尔兹海默症(AD) 佛手柑精油(BEO) 氧化应激 炎症因子 乙酰胆碱酯酶(AchE) 芳香疗法 

分 类 号：R285[医药卫生—中药学] R277.7[医药卫生—中医学]