超高效液相色谱-高分辨质谱联用技术分析地芬尼多代谢产物  

UPLC-HRMS Determining Metabolites of Difenidol from a Poisoning Case

作　　者：王灵 侯建平 WANG Ling;HOU Jianping(Bazhong Public Security Bureau,Bazhong 636000,Sichuan,China)

机构地区：[1]巴中市公安局,四川巴中636000

出　　处：《刑事技术》2022年第1期107-110,共4页Forensic Science and Technology

摘　　要：目的分析地芬尼多在血液中的代谢产物。方法采用高分辨液质联用仪对中毒死亡人员血液进行检测,样品经乙腈沉淀蛋白后使用液相色谱-高分辨质谱联用仪检验,根据高分辨的质谱碎片信息和解析软件分析其结构。结果经过仪器检测和数据分析,推断出血液中三种地芬尼多的代谢产物。结论该方法为地芬尼多中毒案件判定和代谢产物检验提供了新的参考。Objective To determine the metabolites of difenidol in blood of the dead from a poisoning case.Methods Ultra-performance liquid chromatography-high resolution mass spectrometry(UPLC-HRMS)was used to detect the sample from the poisoned dead’s blood that was previously treated with acetonitrile to have the proteins precipitated.The structures of compounds from the sample were to deduce with the information of high-resolution mass-spectral fragments and suitable analysis software.Results Three metabolites of difenidol were identified from UPLC-HRMS detection and relevant data analysis.Conclusion The method established here provides a new reference for detection of metabolites from difenidol and the poisoning cases of the kind.

关 键 词：法医毒物学 超高效液相色谱-高分辨质谱 地芬尼多 代谢产物 

分 类 号：D919.1[医药卫生—法医学]