膜孔径对陶瓷膜净化生物发酵液性能的影响研究  

Study on the effect of pore size of ceramic membrane on the separation performance of biological fermentation broth

作　　者：孟子怡 叶亚晗 余浩卫 闻娟娟 陈献富 邱鸣慧 范益群 MENG Ziyi;YE Yahan;YU Haowei;WEN Juanjuan;CHEN Xianfu;QIU Minghui;FAN Yiqun(State Key Laboratory of Materials-Oriented Chemical Engineering,College of Chemistry and Chemical Engineering,Nanjing Tech University,Nanjing 210009,China)

机构地区：[1]南京工业大学化工学院,材料化学工程国家重点实验室,南京210009

出　　处：《膜科学与技术》2021年第6期95-102,共8页Membrane Science and Technology

基　　金：国家重点研发项目(2021YFC2101200);国家自然科学基金(22078147,21921006);国家合成生物技术创新中心科研项目(TSBICIP-KJGG-002-16);江苏高等教育重点学科建设项目(PAPD);国家级大学生创新创业训练计划项目(2020DC0387)。

摘　　要：生物发酵液具有成分复杂、黏度大、不稳定等特点,膜分离技术应用于发酵液净化时普遍存在有机及生物污染问题,降低了膜的过滤通量,极大限制了膜技术在生物发酵产业的应用.针对发酵液净化工艺中的膜污染问题,提出以小孔径陶瓷超滤膜同时去除菌体、大分子物质和蛋白的方法,探究合适的超滤膜孔径以减小蛋白分子等物质的堵塞污染.研究选取牛血清白蛋白溶液作为基准体系,以增添D-果糖、大肠杆菌种子液配制不同溶液体系,考察了孔径分别为5、10、100 nm 3种陶瓷膜在不同物料体系下的临界运行通量,探究膜孔径与渗透分离性能的构效关系.结果表明,在牛血清白蛋白模拟体系下,孔径为10 nm的小孔径超滤膜表现出更高的临界运行压力和临界运行通量,分别为0.3 MPa和210.26 L/(m^(2)·h);随着料液体系的复杂程度增加,陶瓷膜的临界运行通量逐渐减小,孔径为10 nm的小孔径超滤膜在复杂料液体系中表现出更高的渗透通量和更优的抗污染性能.Biological fouling generally exists in the application of membrane separation technology to fermentation broth treatment due to the characteristics of complex composition,high viscosity,and instability of the biological fermentation broth,which reduces the filtration performance of membranes and greatly limits the application of membrane technology in the biological fermentation industry.This article focuses on the relationship between the membrane pore size and the composition of the target material and liquid,and investigates the critical operating flux of ceramic membranes with different pore sizes of 5~100 nm in different solution systems,and evaluates the effect of the membrane pore size control mechanism on the improvement of the anti-biofouling performance during the application of ceramic membranes.The bovine serum albumin solution was selected as the reference system,and D-fructose and Escherichia coli culture medium were added to prepare different solution systems.Through filtration experiments,the stable permeation flux of ceramic membrane tubes under different pressures was determined.The results show that in the bovine serum albumin simulation system,the critical operating pressure of the 10 nm ceramic membrane tube is about 0.3 MPa,and the critical operating flux is about 210.26 L/(m^(2)·h).The critical operating pressure increases as the pore size decreases.With the complexity of the solution system increases,the critical operating pressure of the ceramic membrane tube gradually decreases,while the ultrafiltration membrane with a small pore size of 10 nm shows better anti-pollution performance in the complex solution system.

关 键 词：抗生物污染 孔径 陶瓷膜 临界运行通量 

分 类 号：TQ028.8[化学工程]