The rice miR171b–SCL6-IIs module controls blast resistance,grain yield,and flowering  

作　　者：Yan Li Ying Tong Xiaorong He Yong Zhu Tingting Li Xiaoyu Lin Wei Mao Zeeshan Ghulam Nabi Gishkori Zhixue Zhao Jiwei Zhang Yanyan Huang Mei Pu Jing Fan Jing Wang Wenming Wang 

机构地区：[1]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Sichuan Agricultural University,Chengdu 611130,Sichuan,China [2]Rice Research Institute and Key Lab for Major Crop Diseases,Sichuan Agricultural University,Chengdu 611130,Sichuan,China

出　　处：《The Crop Journal》2022年第1期117-127,共11页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(U19A2033,31672090,and 31430072);the Sichuan Applied Fundamental Research Foundation(2020YJ0332)to Wenming Wang。

摘　　要：Micro RNAs(mi RNAs)act as regulators of plant development and multiple stress responses.Here we demonstrate that the rice mi R171 b-SCL6-IIs module regulates the balance between blast resistance,grain yield,and flowering.mi R171 b-overexpressing rice plants(OX171 b)displayed increased rice blast resistance accompanied with enhanced defense responses and late heading,whereas blocking mi R171 b expression in rice(MIM171)led to greater susceptibility to blast disease,associated with compromised defense responses and early heading.Either overexpressing or silencing of mi R171 b significantly affected plant height and number of filled seeds per panicle(seed-setting rate),resulting in decreased grain yield.mi R171 b targets SCL6-IIa,SCL6-IIb,and SCL6-IIc,whose expression was suppressed in OX171 b but increased in MIM171.Mutants of SCL6-IIa,SCL6-IIb,and SCL6-IIc all displayed phenotypes like that of OX171 b,including markedly increased blast disease resistance,slightly decreased grain yield,and delayed flowering.Amounts of mi R171 b increased gradually in leaves during the vegetative stage but decreased gradually in panicles during the reproductive stage,whereas SCL6-IIs displayed the reverse expression pattern.Together,these results suggest that the expression of mi R171 b was time-and space-dependent during the rice growth period and regulated the balance between rice blast disease resistance,grain yield,and flowering via SCL6-IIs,and that appropriate accumulation of mi R171 b is essential for rice development.

关 键 词：miR171b SCL6-IIs Blast disease resistance Yield trait FLOWERING 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]