Non-escaping frost tolerant QTL linked genetic loci at reproductive stage in six wheat DH populations  

作　　者：Jingjuan Zhang MD Shahidul Islam Yun Zhao Masood Anwar Zaid Alhabbar Maoyun She Rongchang Yang Angela Juhasz Guixiang Tang Jiansheng Chen Hang Liu Yanjie Jiang Shengnan Zhai Xin Hu Junkang Rong Yingquan Zhang Yebo Qin Qier Liu Zitong Yu Yujuan Zhang Sadegh Balotf Mirza Dowla Sonia Afrin Nandita Roy Md Resad Mallik Md Atik Us Saieed Shanjida Rahman Nigarin Sultana Sarah Al-Sheikh Ahmed Chris Florides Kefei Chen Darshan Sharma Nathan Height Ben Biddulph Meiqin Lu Jorge EMayer Wujun Ma 

机构地区：[1]Australian-China Joint Centre for Wheat Improvement,Western Australian State Agriculture Biotechnology Centre,Food Futures Institute,Murdoch University,South Street,Murdoch,WA 6150,Australia [2]College of Agriculture&Food,Zhejiang Agriculture&Forestry University,Lin’an 311314,Zhejiang,China [3]SAGI West,Faculty of Science and Engineering,Curtin University,Bentley,WA 6102,Australia [4]Department of Primary Industries and Regional Development,Western Australia,3 Baron-Hay Court,South Perth,WA 6151,Australia [5]Australian Grain Technologies,12656 Newell Highway,Locked Bag 1100,Narrabri,NSW 2390,Australia [6]Ag RD&IP Consult P/L,Nerang,QLD 4211,Australia

出　　处：《The Crop Journal》2022年第1期147-165,共19页作物学报（英文版）

基　　金：supported by Murdoch University and the Australia Grains Research&Development Corporation(GRDC)(grant number UMU00048);the Department of Primary Industries and Regional Development(DPIRD),Western Australia;Kalyx Australia Pty Ltd。

摘　　要：Reproductive stage frost poses a major constraint for wheat production in countries such as Australia.However,little progress has been made in identifying key genes to overcome the constraint.In the present study,a severe frost event hit two large-scale field trials consisting of six doubled haploid(DH)wheat populations at reproductive stage(young microspore stage)in Western Australia,leading to the identification of 30 robust frost QTL on 17 chromosomes.The major 18 QTL with the phenotype variation over 9.5%were located on 13 chromosomes including 2 A,2 B,2 D,3 A,4 A,4 B,4 D,5 A,5 D,6 D,7 A,7 B and7 D.Most frost QTL were closely linked to the QTL of anthesis,maturity,Zadok stages as well as linked to anthesis related genes.Out of those,six QTL were repetitively detected on the homologous regions on 2 B,4 B,4 D,5 A,5 D,7 A in more than two populations.Results showed that the frost damage is associated with alleles of Vrn-A1 a,Vrn-D1 a,Rht-B1 b,Rht-D1 b,and the high copy number of Ppd-B1.However,anthesis QTL and anthesis related genes of Vrn-B1 a and Ta FT3-1 B on chromosomes 5 B and 1 B did not lead to frost damage,indicating that these early-flowering phenotype related genes are compatible with frost tolerance and thus can be utilised in breeding.Our results also indicate that wild-type alleles Rht-B1 a and Rht-D1 a can be used when breeding for frost-tolerant varieties without delaying flowering time.

关 键 词：Anthesis-related genes Wheat DH populations Reproductive stage Non-escaping frost tolerance QTL 

分 类 号：S512.1[农业科学—作物学]