A major vernalization-independent QTL for tiller angle on chromosome arm 2BL in bread wheat  

作　　者：Jiajun Liu Jieguang Zhou Huaping Tang Yang Tu Yang Mu Lulu Gou Qiantao Jiang Yaxi Liu Guoyue Chen Jirui Wang Pengfei Qi Wei Li Yunfeng Jiang Zehong Yan Houyang Kang Yuming Wei Xiujin Lan Youliang Zheng Jian Ma 

机构地区：[1]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,Sichuan,China [2]College of Agronomy,Sichuan Agricultural University,Chengdu 611130,Sichuan,China

出　　处：《The Crop Journal》2022年第1期185-193,共9页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31971937 and 31970243);the International Science and Technology Cooperation and Exchanges Program of Science and Technology Department of Sichuan Province(2021YFH0083);the Applied Basic Research Programs of Science and Technology Department of Sichuan Province(2021YJ0503and 2020YJ0140);the Key Projects of Scientific and Technological Activities for Overseas Students of Sichuan Province;the Basic Research Project of Science and Technology Plan of Guizhou Province(ZK2021 general 131)。

摘　　要：Tiller angle(TA)strongly influences plant architecture and grain yield in cereals.However,the genetic basis of TA in wheat is largely unknown.We identified three TA-related quantitative trait loci(QTL).One of them was QTa.sau-2 B-769,a major QTL localized on chromosome arm 2 BL.QTa.sau-2 B-769 was detected in seven environments,explaining 18.1%–51.1%of phenotypic variance.We developed a linked Kompetitive Allele-Specific Polymerase chain reaction(KASP)marker,KASP-AX-108792274,to further validate this locus in three additional populations in multiple environments.QTa.sau-2 B-769 increased TA by up to 24.9%in these populations.There were significant and positive correlations between TA and flag leaf angle(FLANG).However,TA was not correlated with plant height or anthesis date,suggesting that expression of QTa.sau-2 B-769 is independent of vernalization.Traes CS2 B01 G583800,a gene known to be involved in leaf angle regulation,was identified as the most likely candidate gene for QTa.sau-2 B-769.These results enrich our understanding of the mechanisms regulating wheat TA at maturity and may support precise mapping and cloning of gene(s)underlying QTa.sau-2 B-769.

关 键 词：Tiller angle QTL detection and validation Candidate genes Expression patterns 

分 类 号：S512.1[农业科学—作物学]