Calcineurin B-like protein 5(SiCBL5)in Setaria italica enhances salt tolerance by regulating Na^(+)homeostasis  

作　　者：Jingwei Yan Lan Yang Ya Liu Yingdi Zhao Tong Han Xingfen Miao Aying Zhang 

机构地区：[1]College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China [2]State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China [3]College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang,China

出　　处：《The Crop Journal》2022年第1期234-242,共9页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(32001445 and 31871534);the Natural Science Foundation of Jiangsu Province(BK20200557)。

摘　　要：Salinity,a major abiotic stress,reduces plant growth and severely limits agricultural productivity.Plants regulate salt uptake via calcineurin B-like proteins(CBLs).Although extensive studies of the functions of CBLs in response to salt stress have been conducted in Arabidopsis,their functions in Setaria italica are still poorly understood.The foxtail millet genome encodes seven CBLs,of which only SiCBL4 was shown to be involved in salt response.Overexpression of SiCBL5 in Arabidopsis thaliana sos3-1 mutant rescued its salt hypersensitivity phenotype,but that of other SiCBLs(SiCBL1,SiCBL2,SiCBL3,SiCBL6,and SiCBL7)did not rescue the salt hypersensitivity of the Atsos3-1 mutant.SiCBL5 harbors an N-myristoylation motif and is located in the plasma membrane.Overexpression of SiCBL5 in foxtail millet increased its salt tolerance,but its knockdown increased salt hypersensitivity.Yeast two-hybrid and firefly luciferase complementation imaging assays showed that SiCBL5 physically interacted with SiCIPK24 in vitro and in vivo.Cooverexpression of SiCBL5,SiCIPK24,and SiSOS1 in yeast conferred a high-salt-tolerance phenotype.Compared to wild-type plants under salt stress conditions,SiCBL5 overexpressors showed lower accumulations of Na^(+) and stronger Na^(+) efflux,whereas RNAi-SiCBL5 plants showed higher accumulations of Na^(+) and weaker Na^(+) efflux.These results indicate that SiCBL5 confers salt tolerance in foxtail millet by modulating Na^(+) homeostasis.

关 键 词：Setaria italica SiCBL5 Salt tolerance Na^(+)homeostasis 

分 类 号：Q945.78[生物学—植物学]