TaMADS2-3D,a MADS transcription factor gene,regulates phosphate starvation responses in plants  

作　　者：Yingchun Han Na Liu Chuang Li Shuaiwu Wang Lihua Jia Rui Zhang Hui Li Jinfang Tan Hongwei Xue Wenming Zheng 

机构地区：[1]Collaborative Innovation Center of Henan Grain Crops/State Key Laboratory of Wheat and Maize Crop Science,College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,Henan,China [2]College of Resource and Environment,Henan Agricultural University,Zhengzhou 450002,Henan,China [3]School of Agriculture,Sun Yat-sen University,Guangzhou 510275,Guangdong,China [4]School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China

出　　处：《The Crop Journal》2022年第1期243-253,共11页作物学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2018YFD0200600);the Natural Science Foundation of Henan Province(182300410023);the State Key Laboratory of Wheat and Maize Crop Science of China。

摘　　要：Soil inorganic phosphate(Pi)levels are frequently suboptimal for the growth and development of crop plants.Although MADS-box genes participate in diverse plant developmental processes,their involvement in phosphate starvation responses(PSRs)remains unclear.We identified a type I MADS-box transcription factor gene,Ta MADS2-3 D,which was rapidly induced under low-Pi stress in roots of wheat(Triticum aestivum).A Ta MADS2-3 D-GFP fusion protein was found located in the nucleus.Transgenic Arabidopsis plants overexpressing Ta MADS2-3 D(Ta MADS2-3 DOE)showed shortened primary roots,increased lateral root density,and retarded seedling growth under high-Pi(HP)conditions,accompanied by increased Pi contents in their shoots and roots.The Arabidopsis Ta MADS2-3 DOE plants showed similar PSR phenotypes under low Pi(LP)conditions.These results indicate constitutive activation of PSRs by overexpression of Ta MADS2-3 D in Arabidopsis.Reactive oxygen species(ROS),H_(2)O_(2)and O_(2)^(-),levels were increased in root tips of Arabidopsis Ta MADS2-3 DOE plants under HP conditions.Transcriptome analysis of Arabidopsis Ta MADS2-3 DOE plants under different Pi regimes revealed expression changes for a variety of PSR genes including At ZAT6.Overexpression of Ta MADS2-3 D in wheat also led to constitutive activation of PSRs.We propose that Ta MADS2-3 D regulates plant PSRs probably by modulating ROS homeostasis,root development,PSR gene expression,and Pi uptake.This study increases our understanding of plant PSR regulation and provides a valuable gene for improving phosphorus-use efficiency in wheat and other crops.

关 键 词：MADS-box transcription factor Phosphate starvation response WHEAT ARABIDOPSIS 

分 类 号：Q943.2[生物学—植物学]